Antigenic GM3 lactone mimetic molecule integrated mannosylated glycopeptide nanofibers for the activation and maturation of dendritic cells

Date
2017
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Source Title
ACS Applied Materials and Interfaces
Print ISSN
1944-8244
Electronic ISSN
Publisher
American Chemical Society
Volume
9
Issue
19
Pages
16035 - 16042
Language
English
Type
Article
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Abstract

The ability of dendritic cells to coordinate innate and adaptive immune responses makes them essential targets for vaccination strategies. Presentation of specific antigens by dendritic cells is required for the activation of the immune system against many pathogens and tumors, and nanoscale materials can be functionalized for active targeting of dendritic cells. In this work, we integrated an immunogenic, carbohydrate melanoma-associated antigen-mimetic GM3-lactone molecule into mannosylated peptide amphiphile nanofibers to target dendritic cells through DC-SIGN receptor. Based on morphological and functional analyses, when dendritic cells were treated with peptide nanofiber carriers, they showed significant increase in antigen internalization and a corresponding increase in the surface expression of the activation and maturation markers CD86, CD83 and HLA-DR, in addition to exhibiting a general morphology consistent with dendritic cell maturation. These results indicate that mannosylated peptide amphiphile nanofiber carriers are promising candidates to target dendritic cells for antigen delivery. © 2017 American Chemical Society.

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Keywords
Cancer antigens, Carbohydrate melanoma associated antigen, Dendritic cells, Amphiphiles, Antigens, Biomimetics, Carbohydrates, Chemical activation, Cytology, Dermatology, Diseases, Esters, Immune system, Molecules, Nanofibers, Oncology, Peptides, Polypeptides, Adaptive immune response, Antigen delivery, Cancer immunotherapy, Dendritic cells, Nano-scale materials, Peptide amphiphiles, Surface expression, Vaccination strategy, Cells
Citation
Published Version (Please cite this version)