Analysis of apoplastic and symplatsic antioxidant system in shallot leaves: Impacts of weak static electric and magnetic field

Date
2012-07-15
Authors
Cakmak, T.
Cakmak, Z. E.
Dumlupinar, R.
Tekinay, T.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Journal of Plant Physiology
Print ISSN
0176-1617
Electronic ISSN
Publisher
Elsevier
Volume
169
Issue
11
Pages
1066 - 1073
Language
English
Type
Article
Journal Title
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Abstract

Impacts of electric and magnetic fields (EFs and MFs) on a biological organism vary depending on their application style, time, and intensities. High intensity MF and EF have destructive effects on plants. However, at low intensities, these phenomena are of special interest because of the complexity of plant responses. This study reports the effects of continuous, low-intensity static MF (7 mT) and EF (20 kV/m) on growth and antioxidant status of shallot (Allium ascalonicum L.) leaves, and evaluates whether shifts in antioxidant status of apoplastic and symplastic area help plants to adapt a new environment. Growth was induced by MF but EF applied emerged as a stress factor. Despite a lack of visible symptoms of injury, lipid peroxidation and H2O2 levels increased in EF applied leaves. Certain symplastic antioxidant enzyme activities and non-enzymatic antioxidant levels increased in response to MF and EF applications. Antioxidant enzymes in the leaf apoplast, by contrast, were found to show different regulation responses to EF and MF. Our results suggest that apoplastic constituents may work as potentially important redox regulators sensing and signaling environmental changes. Static continuous MF and EF at low intensities have distinct impacts on growth and the antioxidant system in plant leaves, and weak MF is involved in antioxidant-mediated reactions in the apoplast, resulting in overcoming a possible redox imbalance.

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Keywords
Allium ascalonicum, Antioxidant system, Apoplast; Electric field, Magnetic field, ROS
Citation
Published Version (Please cite this version)