Angiogenic peptide nanofibers improve wound healing in STZ-induced diabetic rats

Date
2016-06
Advisor
Instructor
Source Title
ACS Biomaterials Science and Engineering
Print ISSN
2373-9878
Electronic ISSN
Publisher
American Chemical Society
Volume
2
Issue
7
Pages
1180 - 1189
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Low expressions of angiogenic growth factors delay the healing of diabetic wounds by interfering with the process of blood vessel formation. Heparin mimetic peptide nanofibers can bind to and enhance production and activity of major angiogenic growth factors, including VEGF. In this study, we showed that heparin mimetic peptide nanofibers can serve as angiogenic scaffolds that allow slow release of growth factors and protect them from degradation, providing a new therapeutic way to accelerate healing of diabetic wounds. We treated wounds in STZ-induced diabetic rats with heparin mimetic peptide nanofibers and studied repair of full-thickness diabetic skin wounds. Wound recovery was quantified by analyses of re-epithelialization, granulation tissue formation and blood vessel density, as well as VEGF and inflammatory response measurements. Wound closure and granulation tissue formation were found to be significantly accelerated in heparin mimetic gel treated groups. In addition, blood vessel counts and the expressions of alpha smooth muscle actin and VEGF were significantly higher in bioactive gel treated animals. These results strongly suggest that angiogenic heparin mimetic nanofiber therapy can be used to support the impaired healing process in diabetic wounds.

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Other identifiers
Book Title
Keywords
Angiogenesis, Diabetic wound healing, Heparin mimetic, Peptide amphiphiles, VEGF expression, Alpha smooth muscle actin, Angiogenesis inhibitor, Growth factor, Heparin derivative, Heparin mimetic peptide, Nanofiber, Streptozocin, Unclassified drug, Vasculotropin, Animal experiment, Animal tissue, Antiangiogenic therapy, Article, Blood vessel, Capillary density, Controlled study, Diabetic wound, Diabetic wound, Epithelization, Gel, Granulation tissue, Inflammation, Male, Nonhuman, Priority journal, Protein degradation, Protein expression, Rat, Skin, Skinfold thickness, Streptozotocin-induced diabetes mellitus, Wound, Wound closure, Wound healing
Citation
Published Version (Please cite this version)