Spectroscopic evaluation of DNA–borate Interactions
Date
2015
Authors
Ozdemir, A.
Sarioglu O. F.
Tekinay, T.
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Source Title
Biological Trace Element Research
Print ISSN
0163-4984
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Publisher
Humana Press Inc.
Volume
168
Issue
2
Pages
508 - 515
Language
English
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Journal Title
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Abstract
We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV–vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding. © 2015, Springer Science+Business Media New York.
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Keywords
Boron , Calf thymus DNA , Deoxyribose sugar , DNA binding , Spectroscopy , Boric acid , Colemanite , DNA , Sugar phosphate , Ulexite , Unclassified drug , Chemical structure , Circular dichroism , Competitive binding assay , Controlled study , DNA helix , DNA structure , Infrared spectroscopy , Interactions with DNA , Ultraviolet spectroscopy