Ultrasensitive electrospun fluorescent nanofibrous membrane for rapid visual colorimetric detection of H2O2

Source Title
Analytical and Bioanalytical Chemistry
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Electronic ISSN
Springer Verlag
1347 - 1355
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Volume Title

We report herein a flexible fluorescent nanofibrous membrane (FNFM) prepared by decorating the gold nanocluster (AuNC) on electrospun polysulfone nanofibrous membrane for rapid visual colorimetric detection of H2O2. The provision of AuNC coupled to NFM has proven to be advantageous for facile and quick visualization of the obtained results, permitting instant, selective, and on-site detection. We strongly suggest that the fast response time is ascribed to the enhanced probabilities of interaction with AuNC located at the surface of NF. It has been observed that the color change from red to blue is dependent on the concentration, which is exclusively selective for hydrogen peroxide. The detection limit has been found to be 500 nM using confocal laser scanning microscope (CLSM), visually recognizable with good accuracy and stability. A systematic comparison was performed between the sensing performance of FNFM and AuNC solution. The underlying sensing mechanism is demonstrated using UV spectra, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The corresponding disappearance of the characteristic emissions of gold nanoclusters and the emergence of a localized surface plasmon resonance (LSPR) band, stressing this unique characteristic of gold nanoparticles. Hence, it is evident that the conversion of nanoparticles from nanoclusters has taken place in the presence of H2O2. Our work here has paved a new path for the detection of bioanalytes, highlighting the merits of rapid readout, sensitivity, and user-friendliness.

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Book Title
Fluorescence, Gold nanocluster, Hydrogen peroxide, Electrospinning, Nanofiber, Sensor, Color, Colorimetry, Electrospinning, Fluorescence, Gold, High resolution transmission electron microscopy, Hydrogen peroxide, Multilayers, Nanoclusters, Nanofibers, Nanoparticles, Oxidation, Peroxides, Photoelectron spectroscopy, Sensors, Surface plasmon resonance, Transmission electron microscopy, Ultraviolet spectroscopy, Characteristic emission, Confocal laser scanning microscope, Gold nanocluster, Gold Nanoparticles, Localized surface plasmon resonance, Nanofibrous membranes, Sensing performance, Visual colorimetric detections, X ray photoelectron spectroscopy, artificial membrane, Gold, Hydrogen peroxide, Metal nanoparticle, Nanofiber, Artificial membrane, Chemistry, Devices, Electrochemical analysis, Genetic procedures, Infrared spectroscopy, Surface plasmon resonance, Transmission electron microscopy, X ray photoelectron spectroscopy, Biosensing Techniques, Electrochemical Techniques, Gold, Hydrogen Peroxide, Membranes, Artificial, Metal Nanoparticles, Microscopy, Electron, Transmission, Nanofibers, Photoelectron Spectroscopy, Spectroscopy, Fourier Transform Infrared, Surface Plasmon Resonance
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