Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair
Date
2016
Authors
Karahaliloǧlu, Z.
Demirbilek, M.
Şam, M.
Saǧlam, N.
Mizrak, A. K.
Denkbaş, E. B.
Editor(s)
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Artificial Cells, Nanomedicine and Biotechnology
Print ISSN
2169-1401
Electronic ISSN
2169-141X
Publisher
Taylor and Francis Ltd.
Volume
44
Issue
1
Pages
74 - 82
Language
English
Type
Journal Title
Journal ISSN
Volume Title
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Abstract
The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 � 1.50 to 23 � 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.
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Keywords
Bladder reconstruction, Calcium oxalate stones, Nanotexture, Poly [(R)-3-hydroxybutyrate] (PHB), Tissue engineering, Calcium, Glow discharges, Oxalic acid, Tissue, Tissue engineering, Bladder reconstruction, Calcium oxalates, Nano-texture, Plasma modifications, Poly[-3-hydroxybutyrate], Radio frequency glow discharge, Surface-modified, Uroepithelial cells, Scaffolds (biology), 3 hydroxybutyric acid, Calcium, Calcium oxalate, Molecular scaffold, Poly [(r) 3 hydroxybutyrate], Unclassified drug, Biomaterial, Ethylenediamine, Ethylenediamine derivative, Hydroxybutyric acid, Macrogol derivative, Animal cell, Article, Calcium oxalate stone, Cell proliferation, Cell viability, Chemical composition, Contact angle, Crystallization, Electrospinning, Frequency, In vitro study, Nonhuman, Surface property, Tissue repair, Animal, Bladder, Cell line, Cell survival, Chemistry, Cupriavidus necator, Drug effects, Epithelium cell, Isolation and purification, Metabolism, Mouse, Nephrolithiasis, Pathology, Polymerization, Regeneration, Tissue engineering, Animals, Biocompatible materials, Calcium oxalate, Cell line, Cell proliferation, Cell survival, Cupriavidus necator, Epithelial cells, Ethylenediamines, Hydroxybutyrates, Kidney calculi, Mice, Polyethylene glycols, Polymerization, Regeneration, Tissue engineering, Urinary bladder