Label-free optical biodetection of pathogen virulence factors in complex media using microtoroids with multifunctional surface functionality
buir.contributor.author | Bayındır, Mehmet | |
dc.citation.epage | 359 | en_US |
dc.citation.issueNumber | 2 | en_US |
dc.citation.spage | 352 | en_US |
dc.citation.volumeNumber | 3 | en_US |
dc.contributor.author | Toren, P. | en_US |
dc.contributor.author | Ozgur E. | en_US |
dc.contributor.author | Bayındır, Mehmet | en_US |
dc.date.accessioned | 2019-02-21T16:02:28Z | |
dc.date.available | 2019-02-21T16:02:28Z | |
dc.date.issued | 2018 | en_US |
dc.department | Institute of Materials Science and Nanotechnology (UNAM) | en_US |
dc.department | Nanotechnology Research Center (NANOTAM) | en_US |
dc.department | Department of Physics | en_US |
dc.description.abstract | Early detection of pathogens or their virulence factors in complex media has a key role in early diagnosis and treatment of many diseases. Nanomolar and selective detection of Exotoxin A, which is a virulence factor secreted from Pseudomonas aeruginosa in the sputum of Cystic Fibrosis (CF) patients, can pave the way for early diagnosis of P. aeruginosa infections. In this study, we conducted a preliminary study to demonstrate the feasibility of optical biodetection of P. aeruginosa Exotoxin A in a diluted artificial sputum mimicking the CF respiratory environment. Our surface engineering approach provides an effective biointerface enabling highly selective detection of the Exotoxin A molecules in the complex media using monoclonal anti-Exotoxin A functionalized microtoroids. The highly resilient microtoroid surface toward other constituents of the sputum provides Exotoxin A detection ability in the complex media by reproducible measurements. In this study, the limit-of-detection of Exotoxin A in the complex media is calculated as 2.45 nM. | |
dc.description.provenance | Made available in DSpace on 2019-02-21T16:02:28Z (GMT). No. of bitstreams: 1 Bilkent-research-paper.pdf: 222869 bytes, checksum: 842af2b9bd649e7f548593affdbafbb3 (MD5) Previous issue date: 2018 | en |
dc.description.sponsorship | The authors thank to Bulend Ortac (Bilkent University, Turkey) and Caglar Elbuken (Bilkent University, Turkey) for supplying a UV spot curing system and the Tygon tubings, respectively. P.T. would like to thank to Gerburg Schider (Joanneum Research Company, Austria) and Ege Ozgun (Bilkent University, Turkey) for the critic remarks depending on the manuscript. This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with grant no 112T612. | |
dc.identifier.doi | 10.1021/acssensors.7b00775 | en_US |
dc.identifier.issn | 2379-3694 | en_US |
dc.identifier.uri | http://hdl.handle.net/11693/50008 | en_US |
dc.language.iso | English | en_US |
dc.publisher | American Chemical Society | en_US |
dc.relation.isversionof | https://doi.org/10.1021/acssensors.7b00775 | en_US |
dc.relation.project | Bilkent Üniversitesi - 112T612 - Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK | |
dc.source.title | ACS Sensors | en_US |
dc.subject | Biological sensor | en_US |
dc.subject | Cystic fibrosis | en_US |
dc.subject | Label-free detection | en_US |
dc.subject | Microtoroid | en_US |
dc.subject | Optical resonator | en_US |
dc.subject | Surface modification | en_US |
dc.subject | Whispering gallery-mode | en_US |
dc.title | Label-free optical biodetection of pathogen virulence factors in complex media using microtoroids with multifunctional surface functionality | en_US |
dc.type | Article | en_US |
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