Label-free optical biodetection of pathogen virulence factors in complex media using microtoroids with multifunctional surface functionality

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buir.contributor.authorBayındır, Mehmet
dc.citation.epage359en_US
dc.citation.issueNumber2en_US
dc.citation.spage352en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorToren, P.en_US
dc.contributor.authorOzgur E.en_US
dc.contributor.authorBayındır, Mehmeten_US
dc.date.accessioned2019-02-21T16:02:28Z
dc.date.available2019-02-21T16:02:28Z
dc.date.issued2018en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentDepartment of Physicsen_US
dc.description.abstractEarly detection of pathogens or their virulence factors in complex media has a key role in early diagnosis and treatment of many diseases. Nanomolar and selective detection of Exotoxin A, which is a virulence factor secreted from Pseudomonas aeruginosa in the sputum of Cystic Fibrosis (CF) patients, can pave the way for early diagnosis of P. aeruginosa infections. In this study, we conducted a preliminary study to demonstrate the feasibility of optical biodetection of P. aeruginosa Exotoxin A in a diluted artificial sputum mimicking the CF respiratory environment. Our surface engineering approach provides an effective biointerface enabling highly selective detection of the Exotoxin A molecules in the complex media using monoclonal anti-Exotoxin A functionalized microtoroids. The highly resilient microtoroid surface toward other constituents of the sputum provides Exotoxin A detection ability in the complex media by reproducible measurements. In this study, the limit-of-detection of Exotoxin A in the complex media is calculated as 2.45 nM.
dc.description.provenanceMade available in DSpace on 2019-02-21T16:02:28Z (GMT). No. of bitstreams: 1 Bilkent-research-paper.pdf: 222869 bytes, checksum: 842af2b9bd649e7f548593affdbafbb3 (MD5) Previous issue date: 2018en
dc.description.sponsorshipThe authors thank to Bulend Ortac (Bilkent University, Turkey) and Caglar Elbuken (Bilkent University, Turkey) for supplying a UV spot curing system and the Tygon tubings, respectively. P.T. would like to thank to Gerburg Schider (Joanneum Research Company, Austria) and Ege Ozgun (Bilkent University, Turkey) for the critic remarks depending on the manuscript. This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with grant no 112T612.
dc.identifier.doi10.1021/acssensors.7b00775en_US
dc.identifier.issn2379-3694en_US
dc.identifier.urihttp://hdl.handle.net/11693/50008en_US
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttps://doi.org/10.1021/acssensors.7b00775en_US
dc.relation.projectBilkent Üniversitesi - 112T612 - Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK
dc.source.titleACS Sensorsen_US
dc.subjectBiological sensoren_US
dc.subjectCystic fibrosisen_US
dc.subjectLabel-free detectionen_US
dc.subjectMicrotoroiden_US
dc.subjectOptical resonatoren_US
dc.subjectSurface modificationen_US
dc.subjectWhispering gallery-modeen_US
dc.titleLabel-free optical biodetection of pathogen virulence factors in complex media using microtoroids with multifunctional surface functionalityen_US
dc.typeArticleen_US

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