Bio-inspired magnetic beads for isolation of sperm from heterogenous samples ın forensic applications

Date
2021-05
Advisor
Instructor
Source Title
Forensic Science International: Genetics
Print ISSN
1872-4973
Electronic ISSN
Publisher
Elsevier BV
Volume
52
Issue
Pages
102451-1 - 102451-8
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Rapid and efficient processing of sexual assault evidence will accelerate forensic investigation and decrease casework backlogs. The standardized protocols currently used in forensic laboratories require the continued innovation to handle the increasing number and complexity of samples being submitted to forensic labs. Here, we present a new technique leveraging the integration of a bio-inspired oligosaccharide (i.e., Sialyl‐LewisX) with magnetic beads that provides a rapid, inexpensive, and easy-to-use strategy that can potentially be adapted with current differential extraction practice in forensics labs. This platform (i) selectively captures sperm; (ii) is sensitive within the forensic cut-off; (iii) provides a cost effective solution that can be automated with existing laboratory platforms; and (iv) handles small volumes of sample (∼200 μL). This strategy can rapidly isolate sperm within 25 minutes of total processing that will prepare the extracted sample for downstream forensic analysis and ultimately help accelerate forensic investigation and reduce casework backlogs.

Course
Other identifiers
Book Title
Keywords
Sialyl LewisX, Magnetic beads, Bio-inspired material, Cell separation, Forensics
Citation
Published Version (Please cite this version)