Spiral microfluidics device for continuous flow PCR

Date
2013
Advisor
Instructor
Source Title
Proceedings of the ASME 2017 Summer Heat Transfer Conference (HT2017)
Print ISSN
Electronic ISSN
Publisher
ASME
Volume
2
Issue
Pages
Language
English
Type
Conference Paper
Journal Title
Journal ISSN
Volume Title
Abstract

Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL MultiphysicsR . The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications. Copyright © 2013 by ASME.

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Keywords
Biomedical applications, Conventional techniques, Fabrication procedure, Future research directions, Integrated microfluidic devices, Micro-fluidic devices, Microfluidic platforms, Microfluidics devices, Fluidic devices, Fuel cells, Heat transfer, Heating equipment, Mass transfer, Medical applications, Microelectromechanical devices, Microfluidics, Sustainable development, Polymerase chain reaction
Citation
Published Version (Please cite this version)