Synthetic biogenesis of bacterial amyloid nanomaterials with tunable inorganic-organic interfaces and electrical conductivity

Date
2017
Authors
Seker U.O.S.
Chen, A. Y.
Citorik, R. J.
Lu, T. K.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
ACS Synthetic Biology
Print ISSN
2161-5063
Electronic ISSN
Publisher
American Chemical Society
Volume
6
Issue
2
Pages
266 - 275
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

Amyloids are highly ordered, hierarchal protein nanoassemblies. Functional amyloids in bacterial biofilms, such as Escherichia coli curli fibers, are formed by the polymerization of monomeric proteins secreted into the extracellular space. Curli is synthesized by living cells, is primarily composed of the major curlin subunit CsgA, and forms biological nanofibers with high aspect ratios. Here, we explore the application of curli fibers for nanotechnology by engineering curli to mediate tunable biological interfaces with inorganic materials and to controllably form gold nanoparticles and gold nanowires. Specifically, we used cell-synthesized curli fibers as templates for nucleating and growing gold nanoparticles and showed that nanoparticle size could be modulated as a function of curli fiber gold-binding affinity. Furthermore, we demonstrated that gold nanoparticles can be preseeded onto curli fibers and followed by gold enhancement to form nanowires. Using these two approaches, we created artificial cellular systems that integrate inorganic-organic materials to achieve tunable electrical conductivity. We envision that cell-synthesized amyloid nanofibers will be useful for interfacing abiotic and biotic systems to create living functional materials.

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Book Title
Keywords
Biofilm proteins, Functional amyloids, Living materials, Nanomaterial assembly, Amyloid, Bacterial protein, Gold nanoparticle, Gold nanowire, Inorganic compound, Nanomaterial, Nanowire, Organic compound, Unclassified drug, Amyloid, Bacterial protein, Crl protein, Bacteria, Escherichia coli protein, Gold, Metal nanoparticle, Nanofiber, Nanomaterial, Article, Binding affinity, Biogenesis, Chemical engineering, Electric conductivity, Escherichia coli, Metal binding, Nanotechnology, Nonhuman, Particle size, Priority journal, Protein assembly, Protein secondary structure, Transmission electron microscopy, Biofilm, Electric conductivity, Growth, development and aging, Metabolism, Microbiology, Procedures, Amyloid, Bacterial Proteins, Biofilms, Electric Conductivity, Escherichia coli, Escherichia coli Proteins, Gold, Metal Nanoparticles, Nanofibers, Nanostructures, Nanotechnology, Nanowires, Particle Size
Citation
Published Version (Please cite this version)