The role of ligand rebinding and facilitated dissociation on the characterization of dissociation rates by surface plasmon resonance (SPR) and benchmarking performance metrics

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buir.contributor.authorErbaş, Aykut
buir.contributor.authorİnci, Fatih
buir.contributor.orcidErbaş, Aykut|0000-0003-2192-8804
dc.citation.epage253en_US
dc.citation.spage237en_US
dc.citation.volumeNumber2385en_US
dc.contributor.authorErbaş, Aykut
dc.contributor.authorİnci, Fatih
dc.contributor.editorVanhaelen, Quentin
dc.date.accessioned2023-02-15T08:13:15Z
dc.date.available2023-02-15T08:13:15Z
dc.date.issued2022
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractSurface plasmon resonance (SPR) is a real-time kinetic measurement principle that can probe the kinetic interactions between ligands and their binding sites, and lies at the backbone of pharmaceutical, biosensing, and biomolecular research. The extraction of dissociation rates from SPR-response signals often relies on several commonly adopted assumptions, one of which is the exponential decay of the dissociation part of the response signal. However, certain conditions, such as high density of binding sites or high concentration fluctuations near the surface as compared to the bulk, can lead to non-exponential decays via ligand rebinding or facilitated dissociation. Consequently, fitting the data with an exponential function can underestimate or overestimate the measured dissociation rates. Here, we describe a set of alternative fit functions that can take such effects into consideration along with plasmonic sensor design principles with key performance metrics, thereby suggesting methods for error-free high-precision extraction of the dissociation rates.en_US
dc.description.provenanceSubmitted by Evrim Ergin (eergin@bilkent.edu.tr) on 2023-02-15T08:13:15Z No. of bitstreams: 1 The_role_of_ligand_rebinding_and_facilitated_dissociation_on_the_characterization_of_dissociation_rates_by_surface_plasmon_resonance_(SPR)_and_benchmarking_performance_metrics.pdf: 16421794 bytes, checksum: c46f9dfa340e43d094330ee599254e96 (MD5)en
dc.description.provenanceMade available in DSpace on 2023-02-15T08:13:15Z (GMT). No. of bitstreams: 1 The_role_of_ligand_rebinding_and_facilitated_dissociation_on_the_characterization_of_dissociation_rates_by_surface_plasmon_resonance_(SPR)_and_benchmarking_performance_metrics.pdf: 16421794 bytes, checksum: c46f9dfa340e43d094330ee599254e96 (MD5) Previous issue date: 2022en
dc.identifier.doi10.1007/978-1-0716-1767-0_11en_US
dc.identifier.issn1064-3745
dc.identifier.urihttp://hdl.handle.net/11693/111306
dc.language.isoEnglishen_US
dc.publisherSpringeren_US
dc.relation.ispartofComputational methods for estimating the kinetic parameters of biological systemsen_US
dc.relation.ispartofseriesMethods in Molecular Biology;
dc.relation.isversionofhttps://www.doi.org/10.1007/978-1-0716-1767-0_11en_US
dc.source.titleComputational methods for estimating the kinetic parameters of biological systemsen_US
dc.subjectOff-rateen_US
dc.subjectBinding kineticsen_US
dc.subjectFacilitated dissociationen_US
dc.subjectDissociationen_US
dc.subjectPlasmonic sensorsen_US
dc.subjectSurface chemistryen_US
dc.titleThe role of ligand rebinding and facilitated dissociation on the characterization of dissociation rates by surface plasmon resonance (SPR) and benchmarking performance metricsen_US
dc.typeBook Chapteren_US

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