Atmospheric pressure mass spectrometry of single viruses and nanoparticles by nanoelectromechanical systems

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buir.contributor.authorErdogan, R. Tufan
buir.contributor.authorAlkhaled, Mohammed
buir.contributor.authorKaynak, Batuhan E.
buir.contributor.authorAlhmoud, Hashim
buir.contributor.authorPisheh, Hadi Sedaghat
buir.contributor.authorKelleci, Mehmet
buir.contributor.authorKarakurt, Ilbey
buir.contributor.authorŞen, Zehra Betül
buir.contributor.authorHanay, M. Selim
buir.contributor.orcidErdogan, R. Tufan|0000-0001-7657-6362
buir.contributor.orcidAlkhaled, Mohammed|0000-0002-1744-3452
buir.contributor.orcidKaynak, Batuhan E.|0000-0002-7306-4561
buir.contributor.orcidAlhmoud, Hashim|0000-0001-5557-6689
buir.contributor.orcidŞen, Zehra Betül|0000-0002-3897-2329
buir.contributor.orcidHanay, M. Selim|0000-0002-1928-044X
dc.citation.epage3833en_US
dc.citation.issueNumber3en_US
dc.citation.spage3821en_US
dc.citation.volumeNumber16en_US
dc.contributor.authorErdogan, R. Tufan
dc.contributor.authorAlkhaled, Mohammed
dc.contributor.authorKaynak, Batuhan E.
dc.contributor.authorAlhmoud, Hashim
dc.contributor.authorPisheh, Hadi Sedaghat
dc.contributor.authorKelleci, Mehmet
dc.contributor.authorKarakurt, Ilbey
dc.contributor.authorYanik, C.
dc.contributor.authorŞen, Zehra Betül
dc.contributor.authorSari, B.
dc.contributor.authorYagci, A. M.
dc.contributor.authorÖzkul, A.
dc.contributor.authorHanay, M. Selim
dc.date.accessioned2023-02-14T12:26:37Z
dc.date.available2023-02-14T12:26:37Z
dc.date.issued2022-01-04
dc.departmentDepartment of Mechanical Engineeringen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractMass spectrometry of intact nanoparticles and viruses can serve as a potent characterization tool for material science and biophysics. Inaccessible by widespread commercial techniques, the mass of single nanoparticles and viruses (>10MDa) can be readily measured by nanoelectromechanical systems (NEMS)-based mass spectrometry, where charged and isolated analyte particles are generated by electrospray ionization (ESI) in air and transported onto the NEMS resonator for capture and detection. However, the applicability of NEMS as a practical solution is hindered by their miniscule surface area, which results in poor limit-of-detection and low capture efficiency values. Another hindrance is the necessity to house the NEMS inside complex vacuum systems, which is required in part to focus analytes toward the miniscule detection surface of the NEMS. Here, we overcome both limitations by integrating an ion lens onto the NEMS chip. The ion lens is composed of a polymer layer, which charges up by receiving part of the ions incoming from the ESI tip and consequently starts to focus the analytes toward an open window aligned with the active area of the NEMS electrostatically. With this integrated system, we have detected the mass of gold and polystyrene nanoparticles under ambient conditions and with two orders-of-magnitude improvement in capture efficiency compared to the state-of-the-art. We then applied this technology to obtain the mass spectrum of SARS-CoV-2 and BoHV-1 virions. With the increase in analytical throughput, the simplicity of the overall setup, and the operation capability under ambient conditions, the technique demonstrates that NEMS mass spectrometry can be deployed for mass detection of engineered nanoparticles and biological samples efficiently.en_US
dc.identifier.doi10.1021/acsnano.1c08423en_US
dc.identifier.eissn1936-086X
dc.identifier.issn19360851
dc.identifier.urihttp://hdl.handle.net/11693/111264
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/758769/
dc.relation.isversionofhttps://dx.doi.org/10.1021/acsnano.1c08423en_US
dc.relation.projectResonant Electromagnetic Microscopy: Imaging Cells Electronically
dc.rightsinfo:eu-repo/semantics/openAccess
dc.source.titleACS Nanoen_US
dc.subjectNanoelectromechanical systemsen_US
dc.subjectNEMS mass spectrometryen_US
dc.subjectAtmospheric pressure mass spectrometryen_US
dc.subjectLabel-free virus detectionen_US
dc.subjectNanoparticle mass measurementen_US
dc.subjectVacuum-free mass spectrometryen_US
dc.subjectSelf-focusing NEMSen_US
dc.titleAtmospheric pressure mass spectrometry of single viruses and nanoparticles by nanoelectromechanical systemsen_US
dc.typeArticleen_US

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Department of Mechanical Engineering
Institute of Materials Science and Nanotechnology (UNAM)