Supramolecular GAG-like self-assembled glycopeptide nanofibers Induce chondrogenesis and cartilage regeneration

Date
2016
Advisor
Instructor
Source Title
Biomacromolecules
Print ISSN
1525-7797
Electronic ISSN
Publisher
American Chemical Society
Volume
17
Issue
2
Pages
679 - 689
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Glycosaminoglycans (GAGs) and glycoproteins are vital components of the extracellular matrix, directing cell proliferation, differentiation, and migration and tissue homeostasis. Here, we demonstrate supramolecular GAG-like glycopeptide nanofibers mimicking bioactive functions of natural hyaluronic acid molecules. Self-assembly of the glycopeptide amphiphile molecules enable organization of glucose residues in close proximity on a nanoscale structure forming a supramolecular GAG-like system. Our in vitro culture results indicated that the glycopeptide nanofibers are recognized through CD44 receptors, and promote chondrogenic differentiation of mesenchymal stem cells. We analyzed the bioactivity of GAG-like glycopeptide nanofibers in chondrogenic differentiation and injury models because hyaluronic acid is a major component of articular cartilage. Capacity of glycopeptide nanofibers on in vivo cartilage regeneration was demonstrated in microfracture treated osteochondral defect healing. The glycopeptide nanofibers act as a cell-instructive synthetic counterpart of hyaluronic acid, and they can be used in stem cell-based cartilage regeneration therapies.

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Other identifiers
Book Title
Keywords
Cell culture, Cell proliferation, Cells, Cytology, Hyaluronic acid, Molecules, Nanofibers, Organic acids, Peptides, Self assembly, Stem cells, Supramolecular chemistry, Tissue, Tissue homeostasis, Amphiphile molecules, Articular cartilages, Cartilage regeneration, Chondrogenic differentiation, Extracellular matrices, Mesenchymal stem cell, Nanoscale structure, Osteochondral defects, Cartilage, Hermes antigen, Cd44 protein, mouse, Hermes antigen, Cartilage, Mesenchymal Stromal Cells, Molecular Mimicry, Regeneration, Scattering, Small Angle, Tissue Scaffolds, X-Ray Diffraction
Citation
Published Version (Please cite this version)