Basal lamina mimetic nanofibrous peptide networks for skeletal myogenesis

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buir.contributor.authorGüler, Mustafa O.
dc.citation.volumeNumber5en_US
dc.contributor.authorYasa, I. C.en_US
dc.contributor.authorGunduz, N.en_US
dc.contributor.authorKilinc, M.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.date.accessioned2016-02-08T11:00:56Z
dc.date.available2016-02-08T11:00:56Z
dc.date.issued2015en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractExtracellular matrix (ECM) is crucial for the coordination and regulation of cell adhesion, recruitment, differentiation and death. Therefore, equilibrium between cell-cell and cell-matrix interactions and matrix-associated signals are important for the normal functioning of cells, as well as for regeneration. In this work, we describe importance of adhesive signals for myoblast cells' growth and differentiation by generating a novel ECM mimetic peptide nanofiber scaffold system. We show that not only structure but also composition of bioactive signals are important for cell adhesion, growth and differentiation by mimicking the compositional and structural properties of native skeletal muscle basal lamina. We conjugated laminin-derived integrin binding peptide sequence, "IKVAV", and fibronectin-derived well known adhesive sequence, "RGD", into peptide nanostructures to provide adhesive and myogenic cues on a nanofibrous morphology. The myogenic and adhesive signals exhibited a synergistic effect on model myoblasts, C2C12 cells. Our results showed that self-assembled peptide nanofibers presenting laminin derived epitopes support adhesion, growth and proliferation of the cells and significantly promote the expression of skeletal muscle-specific marker genes. The functional peptide nanofibers used in this study present a biocompatible and biodegradable microenvironment, which is capable of supporting the growth and differentiation of C2C12 myoblasts into myotubes.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T11:00:56Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2015en
dc.identifier.doi10.1038/srep16460en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11693/26519
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttps://doi.org/10.1038/srep16460en_US
dc.source.titleScientific Reportsen_US
dc.titleBasal lamina mimetic nanofibrous peptide networks for skeletal myogenesisen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)
Nanotechnology Research Center (NANOTAM)