pH-dependent ionic-current-rectification in nanopipettes modified with glutaraldehyde cross-linked protein membranes

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dc.citation.epage86339en_US
dc.citation.issueNumber89en_US
dc.citation.spage86334en_US
dc.citation.volumeNumber6en_US
dc.contributor.authorŞen, M.en_US
dc.contributor.authorDemirci, A.en_US
dc.date.accessioned2018-04-12T10:48:05Z
dc.date.available2018-04-12T10:48:05Z
dc.date.issued2016en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractIn this study, we investigated for the first time the influence of an artificial membrane on the ionic current rectification of nanopipettes at various pH levels. The nanopipettes were fabricated and then modified with bovine serum albumin-glutaraldehyde (BSA-GA) artificial membranes. We determined the degree of ionic current rectification of these nanopipettes and compared them with those of bare nanopipettes. In contrast to the bare nanopipettes, the BSA-GA-modified nanopipettes demonstrated pH-dependent ionic current rectification. We also examined the tunability of the degree of rectification using streptavidin (STV) whose isoelectric point differs from that of BSA. The results showed that the ionic current rectification of nanopipettes can be tuned as the addition of STV into the BSA-GA artificial membrane increases the degree of rectification. Using the proposed approach, nanoscale spearhead pH sensors could be fabricated for highly localized extracellular or intracellular pH measurement. Moreover, it is possible to realize the applications of nano-sized channels in relatively larger channels using the present method.en_US
dc.identifier.doi10.1039/c6ra19263gen_US
dc.identifier.eissn2046-2069en_US
dc.identifier.urihttp://hdl.handle.net/11693/36676
dc.language.isoEnglishen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionofhttp://dx.doi.org/10.1039/c6ra19263gen_US
dc.source.titleRSC Advancesen_US
dc.subjectBody fluidsen_US
dc.subjectEnzyme activityen_US
dc.subjectpHen_US
dc.subjectpH sensorsen_US
dc.subjectProteinsen_US
dc.subjectArtificial membranesen_US
dc.subjectBovine serum albuminsen_US
dc.subjectCross-linked proteinsen_US
dc.subjectExtracellularen_US
dc.subjectGlutaraldehydesen_US
dc.subjectIntracellular pH measurementen_US
dc.subjectIonic current rectificationsen_US
dc.subjectIso-electric pointsen_US
dc.subjectElectric rectifiersen_US
dc.titlepH-dependent ionic-current-rectification in nanopipettes modified with glutaraldehyde cross-linked protein membranesen_US
dc.typeArticleen_US

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