Impedance-based viscoelastic flow cytometry

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buir.contributor.authorSerhatlıoğlu, Murat
buir.contributor.authorAsghari, Mohammad
buir.contributor.authorElbuken, Çağlar
dc.citation.epage913en_US
dc.citation.issueNumber6en_US
dc.citation.spage906en_US
dc.citation.volumeNumber40en_US
dc.contributor.authorSerhatlıoğlu, Muraten_US
dc.contributor.authorAsghari, Mohammaden_US
dc.contributor.authorGuler, M. T.en_US
dc.contributor.authorElbuken, Çağlaren_US
dc.date.accessioned2020-02-10T11:22:11Z
dc.date.available2020-02-10T11:22:11Z
dc.date.issued2019
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractElastic nature of the viscoelastic fluids induces lateral migration of particles into a single streamline and can be used by microfluidic based flow cytometry devices. In this study, we investigated focusing efficiency of polyethylene oxide based viscoelastic solutions at varying ionic concentration to demonstrate their use in impedimetric particle characterization systems. Rheological properties of the viscoelastic fluid and particle focusing performance are not affected by ionic concentration. We investigated the viscoelastic focusing dynamics using polystyrene (PS) beads and human red blood cells (RBCs) suspended in the viscoelastic fluid. Elasto‐inertial focusing of PS beads was achieved with the combination of inertial and viscoelastic effects. RBCs were aligned along the channel centerline in parachute shape which yielded consistent impedimetric signals. We compared our impedance‐based microfluidic flow cytometry results for RBCs and PS beads by analyzing particle transit time and peak amplitude at varying viscoelastic focusing conditions obtained at different flow rates. We showed that single orientation, single train focusing of nonspherical RBCs can be achieved with polyethylene oxide based viscoelastic solution that has been shown to be a good candidate as a carrier fluid for impedance cytometry.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey. Grant Number: 215E086en_US
dc.embargo.release2020-03-12
dc.identifier.doi10.1002/elps.201800365en_US
dc.identifier.issn0173-0835
dc.identifier.urihttp://hdl.handle.net/11693/53228
dc.language.isoEnglishen_US
dc.publisherWILEY‐VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.relation.isversionofhttps://doi.org/10.1002/elps.201800365en_US
dc.source.titleElectrophoresisen_US
dc.subjectImpedance cytometryen_US
dc.subjectMicrofluidicsen_US
dc.subjectRheologyen_US
dc.subjectSingle cell characterizationen_US
dc.subjectViscoelastic focusingen_US
dc.titleImpedance-based viscoelastic flow cytometryen_US
dc.typeArticleen_US

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