Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses
dc.citation.issueNumber | 8 | en_US |
dc.citation.volumeNumber | 3 | en_US |
dc.contributor.author | Plagnol V. | en_US |
dc.contributor.author | Uz, E. | en_US |
dc.contributor.author | Wallace, C. | en_US |
dc.contributor.author | Stevens H. | en_US |
dc.contributor.author | Clayton, D. | en_US |
dc.contributor.author | Ozcelik, T. | en_US |
dc.contributor.author | Todd J.A. | en_US |
dc.date.accessioned | 2016-02-08T10:08:06Z | |
dc.date.available | 2016-02-08T10:08:06Z | |
dc.date.issued | 2008 | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.department | Institute of Materials Science and Nanotechnology (UNAM) | en_US |
dc.description.abstract | Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and (genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays. © 2008 Plagnol et al. | en_US |
dc.description.provenance | Made available in DSpace on 2016-02-08T10:08:06Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2008 | en |
dc.identifier.doi | 10.1371/journal.pone.0002966 | en_US |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | http://hdl.handle.net/11693/23035 | |
dc.language.iso | English | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pone.0002966 | en_US |
dc.source.title | PLoS ONE | en_US |
dc.subject | adult | en_US |
dc.subject | allele | en_US |
dc.subject | allelic imbalance | en_US |
dc.subject | article | en_US |
dc.subject | child | en_US |
dc.subject | clonal variation | en_US |
dc.subject | controlled study | en_US |
dc.subject | female | en_US |
dc.subject | gene expression profiling | en_US |
dc.subject | genome analysis | en_US |
dc.subject | human | en_US |
dc.subject | human cell | en_US |
dc.subject | insulin dependent diabetes mellitus | en_US |
dc.subject | lymphoblastoid cell line | en_US |
dc.subject | major clinical study | en_US |
dc.subject | newborn | en_US |
dc.subject | X chromosome inactivation | en_US |
dc.subject | blood cell | en_US |
dc.subject | cell clone | en_US |
dc.subject | cell culture | en_US |
dc.subject | cell line | en_US |
dc.subject | cytology | en_US |
dc.subject | gene expression | en_US |
dc.subject | genetics | en_US |
dc.subject | immunology | en_US |
dc.subject | lymphocyte | en_US |
dc.subject | physiology | en_US |
dc.subject | quantitative trait locus | en_US |
dc.subject | reference value | en_US |
dc.subject | single nucleotide polymorphism | en_US |
dc.subject | RNA | en_US |
dc.subject | Blood Cells | en_US |
dc.subject | Cell Line | en_US |
dc.subject | Cells, Cultured | en_US |
dc.subject | Clone Cells | en_US |
dc.subject | Female | en_US |
dc.subject | Gene Expression | en_US |
dc.subject | Humans | en_US |
dc.subject | Lymphocytes | en_US |
dc.subject | Polymorphism, Single Nucleotide | en_US |
dc.subject | Quantitative Trait Loci | en_US |
dc.subject | Reference Values | en_US |
dc.subject | RNA | en_US |
dc.title | Extreme clonality in lymphoblastoid cell lines with implications for allele specific expression analyses | en_US |
dc.type | Article | en_US |
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