Evaluation of chimerism with DNA polymorphisms in bone marrow transplantation

Date
1997
Authors
Özbek, U.
Vural, B.
Kalayoǧlu, S.
Soysal, T.
Bilgen, H.
Yavuz, S.
Anak, S.
Sargın, D.
Gedikoǧlu G.
Ferhanoǧlu, Burhan
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Turkish Journal of Pediatrics
Print ISSN
0041-4301
Electronic ISSN
Publisher
International Children's Center (I C C)
Volume
39
Issue
3
Pages
303 - 311
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

Evaluation of chimeric status following allogenlc BMT is an Important tool for monitoring the replacement of host cells with donor cells and for determining the risk of relapse. Polymorphic DNA sequences can be used as powerful markers in identification of donor/recipient genotype differences, even between close relatives. Polymerase chain reaction (PCR) amplification of three variable number of tandem repeat (VNTR) loci and five single-locus polymorphisms (SLP) was used to identify chimerism in 40 recipient-donor pairs. Mixed chimerism was present in 11 patients, and complete chimerism in 29. This PCR method is a rapid and sensitive assay to detect engraftment and evaluate relapse potential, and thus is very useful in the clinical management of BMT patients.

Course
Other identifiers
Book Title
Keywords
Bone marrow transplantation, Chimerism, Polymerase chain reaction, Polymorphism, Relapse, VNTR, Adolescent, Adult, Bone marrow transplantation, Child, Chimera, Female, Genetic marker, Genetic polymorphism, Preschool child, Variable number of tandem repeat, Adolescent, Child, Preschool, Chimera, Genetic Markers, Humans, Male, Minisatellite Repeats, Polymorphism, genetic
Citation
Published Version (Please cite this version)