Chondrogenic differentiation of mesenchymal stem cells on glycosaminoglycan-mimetic peptide nanofibers

dc.citation.epage878en_US
dc.citation.issueNumber5en_US
dc.citation.spage871en_US
dc.citation.volumeNumber2en_US
dc.contributor.authorYaylaci, S .U.en_US
dc.contributor.authorSen, M.en_US
dc.contributor.authorBulut, O.en_US
dc.contributor.authorArslan, E.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.contributor.bilkentauthorGüler, Mustafa O.
dc.date.accessioned2018-04-12T10:50:53Z
dc.date.available2018-04-12T10:50:53Z
dc.date.issued2016en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractGlycosaminoglycans (GAGs) are important extracellular matrix components of cartilage tissue and provide biological signals to stem cells and chondrocytes for development and functional regeneration of cartilage. Among their many functions, particularly sulfated glycosaminoglycans bind to growth factors and enhance their functionality through enabling growth factor-receptor interactions. Growth factor binding ability of the native sulfated glycosaminoglycans can be incorporated into the synthetic scaffold matrix through functionalization with specific chemical moieties. In this study, we used peptide amphiphile nanofibers functionalized with the chemical groups of native glycosaminoglycan molecules such as sulfonate, carboxylate and hydroxyl to induce the chondrogenic differentiation of rat mesenchymal stem cells (MSCs). The MSCs cultured on GAG-mimetic peptide nanofibers formed cartilage-like nodules and deposited cartilage-specific matrix components by day 7, suggesting that the GAG-mimetic peptide nanofibers effectively facilitated their commitment into the chondrogenic lineage. Interestingly, the chondrogenic differentiation degree was manipulated with the sulfonation degree of the nanofiber system. The GAG-mimetic peptide nanofibers network presented here serve as a tailorable bioactive and bioinductive platform for stem-cell-based cartilage regeneration studies.en_US
dc.identifier.doi10.1021/acsbiomaterials.6b00099en_US
dc.identifier.issn2373-9878
dc.identifier.urihttp://hdl.handle.net/11693/36724
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/acsbiomaterials.6b00099en_US
dc.source.titleACS Biomaterials Science and Engineeringen_US
dc.subjectChondrogenic differentiationen_US
dc.subjectGAG-mimeticen_US
dc.subjectIn vitro condensationen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectPeptide amphiphile nanofiberen_US
dc.subjectAggrecanen_US
dc.subjectAmphophileen_US
dc.subjectCarboxylic aciden_US
dc.subjectCollagen type 1en_US
dc.subjectCollagen type 2en_US
dc.subjectEpitopeen_US
dc.subjectGlycosaminoglycanen_US
dc.subjectGlycosaminoglycan polysulfateen_US
dc.subjectHydroxyl groupen_US
dc.subjectNanofiberen_US
dc.subjectAnimal cellen_US
dc.subjectAnimal experimenten_US
dc.subjectBiological activityen_US
dc.subjectCartilageen_US
dc.subjectCell aggregationen_US
dc.subjectCell componenten_US
dc.subjectCell cultureen_US
dc.subjectCell differentiationen_US
dc.subjectCell functionen_US
dc.subjectChemical analysisen_US
dc.subjectChondrogenesisen_US
dc.subjectChondrogenic differentiationen_US
dc.subjectClinical effectivenessen_US
dc.subjectExtracellular matrixen_US
dc.subjectGene expression profilingen_US
dc.subjectMesenchymal stem cellen_US
dc.subjectNonhumanen_US
dc.titleChondrogenic differentiation of mesenchymal stem cells on glycosaminoglycan-mimetic peptide nanofibersen_US
dc.typeArticleen_US
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