Investigation of binding properties of dicationic styrylimidazo[1,2-a]pyridinium dyes to human serum albumin by spectroscopic techniques

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dc.citation.epage92en_US
dc.citation.issueNumber1en_US
dc.citation.spage86en_US
dc.citation.volumeNumber32en_US
dc.contributor.authorÖzdemir, A.en_US
dc.contributor.authorGökoğlu, E.en_US
dc.contributor.authorYılmaz, Esraen_US
dc.contributor.authorYalçın, E.en_US
dc.contributor.authorGökoğlu, E.en_US
dc.contributor.authorSeferoğlu, Z.en_US
dc.contributor.authorTekinay, T.en_US
dc.date.accessioned2018-04-12T10:39:10Z
dc.date.available2018-04-12T10:39:10Z
dc.date.issued2017en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractThe binding interaction between two dicationic styrylimidazo[1,2-a]pyridinium dyes and human serum albumin (HSA) was investigated at physiological conditions using fluorescence, UV–vis absorption, and circular dichroism (CD) spectroscopies. Analysis of the fluorescence titration data at different temperatures suggested that the fluorescence quenching mechanism of HSA by these dyes was static. The calculated thermodynamic parameters (ΔG°, ΔH° and ΔS°) indicated that hydrogen bonding and van der Waals forces played a major role in the formation of the dye–HSA complex. Binding distances (r) between dyes and HSA were calculated according to Förster's non-radiative energy transfer theory. Studies of conformational changes of HSA using CD measurements indicate that the α-helical content of the protein decreased upon binding of the dyes.en_US
dc.embargo.release2018-01-08en_US
dc.identifier.doi10.1002/bio.3153en_US
dc.identifier.issn1522-7235
dc.identifier.urihttp://hdl.handle.net/11693/36418
dc.language.isoEnglishen_US
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/bio.3153en_US
dc.source.titleLuminescenceen_US
dc.subjectBinding modeen_US
dc.subjectCircular dichroismen_US
dc.subjectDicationic styryl dyesen_US
dc.subjectFluorescence quenchingen_US
dc.subjectHuman serum albuminen_US
dc.subjectCationen_US
dc.subjectFluorescent dyeen_US
dc.subjectImidazole derivativeen_US
dc.subjectPyridinium derivativeen_US
dc.subjectSerum albuminen_US
dc.subjectStyrylimidazo(1,2-a)pyridiniumen_US
dc.subjectBinding siteen_US
dc.subjectChemistryen_US
dc.subjectCircular dichroismen_US
dc.subjectHumanen_US
dc.subjectSpectrofluorometryen_US
dc.subjectThermodynamicsen_US
dc.subjectUltraviolet spectrophotometryen_US
dc.subjectBinding Sitesen_US
dc.subjectCationsen_US
dc.subjectCircular Dichroismen_US
dc.subjectFluorescent Dyesen_US
dc.subjectImidazolesen_US
dc.subjectPyridinium Compoundsen_US
dc.subjectSerum Albuminen_US
dc.subjectSpectrometryen_US
dc.subjectSpectrophotometryen_US
dc.subjectThermodynamicsen_US
dc.subjectUltravioleten_US
dc.subjectFluorescenceen_US
dc.titleInvestigation of binding properties of dicationic styrylimidazo[1,2-a]pyridinium dyes to human serum albumin by spectroscopic techniquesen_US
dc.typeArticleen_US

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