Computer experiments to determine whether over-or under-counting necessarily affects the determination of difference in cell number between experimental groups

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dc.citation.epage99en_US
dc.citation.issueNumber1en_US
dc.citation.spage91en_US
dc.citation.volumeNumber91en_US
dc.contributor.authorHeller, B.en_US
dc.contributor.authorSchweingruber, F.en_US
dc.contributor.authorGuvenc, D.en_US
dc.contributor.authorHeller, A.en_US
dc.date.accessioned2015-07-28T11:56:23Z
dc.date.available2015-07-28T11:56:23Z
dc.date.issued2001-03-30en_US
dc.departmentDepartment of Mathematicsen_US
dc.description.abstractComputer cell counting experiments were performed in order to examine the consequences of over- or under-counting. The three-dimensional reaggregate culture laboratory environment for cell counting was used as a model for computer simulation. The laboratory environment for aggregate and cell sizes, numbers and spatial placement in gelatin blocks was mimicked in the computer setup. However, in the computer, cell counting was set to be either ideally unbiased, or deliberately biased in regard to over- or under-counting so as to compare eventual results when using the various cell counting methods. It was found that there was no effect of the cell counting methods used in determining whether there was a significant difference in cell number between two experimental groups. In addition, it was found that under the conditions of these simulations, the optical disector method behaved similarly, on the average, as the ideal method of counting cell centers and in both of those cases, the average ratio between actual cell number in a flask and estimated number was close to 1.00. © 2001 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.doi10.1016/S0165-0270(01)00333-8en_US
dc.identifier.eissn1872-678X
dc.identifier.issn0165-0270
dc.identifier.urihttp://hdl.handle.net/11693/10947
dc.language.isoEnglishen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/S0165-0270(01)00333-8en_US
dc.source.titleJournal of Neuroscience Methodsen_US
dc.subjectCell countingen_US
dc.subjectComputer simulationen_US
dc.subjectCell cultureen_US
dc.subjectDopaminergic neuronsen_US
dc.subjectReaggregatesen_US
dc.subjectDopamineen_US
dc.subjectOptical disectoren_US
dc.titleComputer experiments to determine whether over-or under-counting necessarily affects the determination of difference in cell number between experimental groupsen_US
dc.typeArticleen_US

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