A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus
dc.citation.epage | 33 | en_US |
dc.citation.issueNumber | 1 | en_US |
dc.citation.spage | 29 | en_US |
dc.citation.volumeNumber | 44 | en_US |
dc.contributor.author | Eroğlu, C. | en_US |
dc.contributor.author | Yıldız, E. | en_US |
dc.contributor.author | Öztürk, M. | en_US |
dc.contributor.author | Pınarbaşı, E. | en_US |
dc.date.accessioned | 2016-02-08T10:37:28Z | |
dc.date.available | 2016-02-08T10:37:28Z | |
dc.date.issued | 2000 | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.description.abstract | In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera. | en_US |
dc.description.provenance | Made available in DSpace on 2016-02-08T10:37:28Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2000 | en |
dc.identifier.eissn | 1336-2305 | en_US |
dc.identifier.issn | 0001-723X | |
dc.identifier.uri | http://hdl.handle.net/11693/24999 | |
dc.language.iso | English | en_US |
dc.source.title | Acta Virologica | en_US |
dc.subject | Cloning | en_US |
dc.subject | Core protein gene | en_US |
dc.subject | ELISA | en_US |
dc.subject | Expression | en_US |
dc.subject | Hepatitis C virus | en_US |
dc.subject | Nucleotide sequencing | en_US |
dc.subject | Purification | en_US |
dc.subject | RT-PCR | en_US |
dc.subject | Complementary DNA | en_US |
dc.subject | Core protein | en_US |
dc.subject | Hepatitis C antibody | en_US |
dc.subject | RNA | en_US |
dc.subject | Serologic Tests | en_US |
dc.subject | Viral Core Proteins | en_US |
dc.subject | Escherichia coli | en_US |
dc.title | A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus | en_US |
dc.type | Article | en_US |
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