A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus

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dc.citation.epage33en_US
dc.citation.issueNumber1en_US
dc.citation.spage29en_US
dc.citation.volumeNumber44en_US
dc.contributor.authorEroğlu, C.en_US
dc.contributor.authorYıldız, E.en_US
dc.contributor.authorÖztürk, M.en_US
dc.contributor.authorPınarbaşı, E.en_US
dc.date.accessioned2016-02-08T10:37:28Z
dc.date.available2016-02-08T10:37:28Z
dc.date.issued2000en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractIn this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.en_US
dc.identifier.eissn1336-2305en_US
dc.identifier.issn0001-723X
dc.identifier.urihttp://hdl.handle.net/11693/24999
dc.language.isoEnglishen_US
dc.source.titleActa Virologicaen_US
dc.subjectCloningen_US
dc.subjectCore protein geneen_US
dc.subjectELISAen_US
dc.subjectExpressionen_US
dc.subjectHepatitis C virusen_US
dc.subjectNucleotide sequencingen_US
dc.subjectPurificationen_US
dc.subjectRT-PCRen_US
dc.subjectComplementary DNAen_US
dc.subjectCore proteinen_US
dc.subjectHepatitis C antibodyen_US
dc.subjectRNAen_US
dc.subjectSerologic Testsen_US
dc.subjectViral Core Proteinsen_US
dc.subjectEscherichia colien_US
dc.titleA highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virusen_US
dc.typeArticleen_US

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