A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus
Date
2000
Authors
Eroğlu, C.
Yıldız, E.
Öztürk, M.
Pınarbaşı, E.
Editor(s)
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Acta Virologica
Print ISSN
0001-723X
Electronic ISSN
1336-2305
Publisher
Volume
44
Issue
1
Pages
29 - 33
Language
English
Type
Journal Title
Journal ISSN
Volume Title
Series
Abstract
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.