A recombinase-based genetic circuit for heavy metal monitoring

buir.contributor.orcidSaltepe, Behide|0000-0002-4338-6463en_US
buir.contributor.orcidAkboğa, Doğuş|0000-0002-0426-6135en_US
dc.citation.epage10en_US
dc.citation.issueNumber2en_US
dc.citation.spage1en_US
dc.citation.volumeNumber12en_US
dc.contributor.authorAkboğa, Doğuş
dc.contributor.authorSaltepe, Behide
dc.contributor.bilkentauthorAkboğa, Doğuş
dc.contributor.bilkentauthorSaltepe, Behide
dc.date.accessioned2023-03-02T11:44:33Z
dc.date.available2023-03-02T11:44:33Z
dc.date.issued2022-02-16
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractRapid progress in the genetic circuit design enabled whole-cell biosensors (WCBs) to become prominent in detecting an extensive range of analytes with promise in many fields, from medical diagnostics to environmental toxicity assessment. However, several drawbacks, such as high background signal or low precision, limit WCBs to transfer from proof-of-concept studies to real-world applications, particularly for heavy metal toxicity monitoring. For an alternative WCB module design, we utilized Bxb1 recombinase that provides tight control as a switch to increase dose-response behavior concerning leakiness. The modularity of Bxb1 recombinase recognition elements allowed us to combine an engineered semi-specific heat shock response (HSR) promoter, sensitive to stress conditions including toxic ions such as cadmium, with cadmium resistance regulatory elements; a cadmium-responsive transcription factor and its cognitive promoter. We optimized the conditions for the recombinase-based cadmium biosensor to obtain increased fold change and shorter response time. This system can be expanded for various heavy metals to make an all-in-one type of WCB, even using semi-specific parts of a sensing system. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.en_US
dc.identifier.doi10.3390/bios12020122en_US
dc.identifier.issn20796374
dc.identifier.urihttp://hdl.handle.net/11693/112020
dc.language.isoEnglishen_US
dc.publisherMDPIen_US
dc.relation.isversionofhttps://dx.doi.org/10.3390/bios12020122en_US
dc.source.titleBiosensorsen_US
dc.subjectBxb1 recombinaseen_US
dc.subjectHeavy metal detectionen_US
dc.subjectSynthetic biologyen_US
dc.subjectToxicityen_US
dc.subjectWhole-cell biosensoren_US
dc.titleA recombinase-based genetic circuit for heavy metal monitoringen_US
dc.typeArticleen_US
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