Localized Surface Plasmon Resonance (LSPR) is based on the electromagnetic-field enhancement of metallic nano-particles. It is observed at the metal-dielectric interface and the resonance wavelength can be tuned by the size, shape, and periodicity of the metallic nanoparticles and the surrounding dielectric environment. This makes LSPR a powerful candidate in bio-sensing. In the present work, the size and period dependency of the LSPR wavelength was studied through simulations and fabrications. The surface functionalization, that transforms the surface into a sensing platform was done and verified. Finally, the concentration dependency of the LSPR shifts was observed. All the measurements were done by a transmission set-up. The study is at an early stage, however results are promising. The detection of specific bacteria species can be made possible with such a detection method. © 2011 SPIE.