Sheathless microflow cytometry using viscoelastic fluids

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dc.citation.epage14en_US
dc.citation.issueNumber12342en_US
dc.citation.spage1en_US
dc.citation.volumeNumber7en_US
dc.contributor.authorAsghari, M.en_US
dc.contributor.authorSerhatlioglu, M.en_US
dc.contributor.authorOrtaƧ, B.en_US
dc.contributor.authorSolmaz, M. E.en_US
dc.contributor.authorElbuken, C.en_US
dc.date.accessioned2018-04-12T11:07:42Z
dc.date.available2018-04-12T11:07:42Z
dc.date.issued2017en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractMicroflow cytometry is a powerful technique for characterization of particles suspended in a solution. In this work, we present a microflow cytometer based on viscoelastic focusing. 3D single-line focusing of microparticles was achieved in a straight capillary using viscoelastic focusing which alleviated the need for sheath flow or any other actuation mechanism. Optical detection was performed by fiber coupled light source and photodetectors. Using this system, we present the detection of microparticles suspended in three different viscoelastic solutions. The rheological properties of the solutions were measured and used to assess the focusing performance both analytically and numerically. The results were verified experimentally, and it has been shown that polyethlyene oxide (PEO) and hyaluronic acid (HA) based sheathless microflow cytometer demonstrates similar performance to state-of-the art flow cytometers. The sheathless microflow cytometer was shown to present 780 particles/s throughput and 5.8% CV for the forward scatter signal for HA-based focusing. The presented system is composed of a single capillary to accommodate the fluid and optical fibers to couple the light to the fluid of interest. Thanks to its simplicity, the system has the potential to widen the applicability of microflow cytometers.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T11:07:42Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017en
dc.identifier.doi10.1038/s41598-017-12558-2en_US
dc.identifier.eissn2045-2322en_US
dc.identifier.urihttp://hdl.handle.net/11693/37262
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41598-017-12558-2en_US
dc.source.titleScientific Reportsen_US
dc.subjectBiomedical engineeringen_US
dc.subjectOptical materials and structuresen_US
dc.titleSheathless microflow cytometry using viscoelastic fluidsen_US
dc.typeArticleen_US

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