The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits

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buir.contributor.authorShirejini, Saeedreza Zeibi
buir.contributor.authorİnci, Fatih
buir.contributor.orcidShirejini, Saeedreza Zeibi|0000-0003-4403-335X
buir.contributor.orcidİnci, Fatih|0000-0002-9918-5038
dc.citation.epage107814-19en_US
dc.citation.spage107814-1en_US
dc.citation.volumeNumber54en_US
dc.contributor.authorShirejini, Saeedreza Zeibi
dc.contributor.authorİnci, Fatih
dc.date.accessioned2022-01-26T11:58:04Z
dc.date.available2022-01-26T11:58:04Z
dc.date.issued2022-01
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractExosomes are a subset of extracellular vesicles released from various cells, and they can be found in different bodily fluids. Exosomes have been utilized as biomarkers to diagnose many diseases and to monitor therapy efficiency as they represent the status and origin of the cell, which they are released from. Considering that they co-exist in bodily fluids with other types of particles, their isolation still remains challenging since conventional methods are time-consuming, user-dependent, and result in low isolation yield. This review summarizes the conventional strategies and microfluidic-based methods for exosome isolation along with their strengths and limitations. In particular, microfluidic devices emerge as a promising approach to tackle the existing limitations of conventional methods, and they provide unique features, such as operating with minute volume of samples and rapid process, in order to isolate exosomes with the high yield and the high purity, which make them unprecedented tools for molecular biology and clinical applications in exosome research. This review further elaborates on the existing microfluidic-based exosome isolation methods and denotes their benefits and drawbacks. Herein, we also introduce various commercially available platforms and kits for exosome isolation along with their working principles.en_US
dc.embargo.release2023-01-28
dc.identifier.doi10.1016/j.biotechadv.2021.107814en_US
dc.identifier.eissn1873-1899
dc.identifier.issn0734-9750
dc.identifier.urihttp://hdl.handle.net/11693/76802
dc.language.isoEnglishen_US
dc.publisherElsevier Incen_US
dc.relation.isversionofhttps://doi.org/10.1016/j.biotechadv.2021.107814en_US
dc.source.titleBiotechnology Advancesen_US
dc.subjectMicrofluidicsen_US
dc.subjectExosomeIsolation methodsen_US
dc.subjectBiomarkeren_US
dc.subjectExtracellular vesiclesen_US
dc.subjectLab-on-a-chipen_US
dc.subjectCommercial kitsen_US
dc.titleThe Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kitsen_US
dc.typeReviewen_US
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