Dentin phosphoprotein mimetic peptide nanofibers promote biomineralization

Date
2019
Advisor
Instructor
Source Title
Macromolecular Bioscience
Print ISSN
1616-5187
Electronic ISSN
Publisher
WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Volume
19
Issue
1
Pages
1800080-1 - 1800080-10
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

Dentin phosphoprotein (DPP) is a major component of the dentin matrix playing crucial role in hydroxyapatite deposition during bone mineralization, making it a prime candidate for the design of novel materials for bone and tooth regeneration. The bioactivity of DPP‐derived proteins is controlled by the phosphorylation and dephosphorylation of the serine residues. Here an enzyme‐responsive peptide nanofiber system inducing biomineralization is demonstrated. It closely emulates the structural and functional properties of DPP and facilitates apatite‐like mineral deposition. The DPP‐mimetic peptide molecules self‐assemble through dephosphorylation by alkaline phosphatase (ALP), an enzyme participating in tooth and bone matrix mineralization. Nanofiber network formation is also induced through addition of calcium ions. The gelation process following nanofiber formation produces a mineralized extracellular matrix like material, where scaffold properties and phosphate groups promote mineralization. It is demonstrated that the DPP‐mimetic peptide nanofiber networks can be used for apatite‐like mineral deposition for bone regeneration.

Course
Other identifiers
Book Title
Keywords
Biomineralization, Dentin phosphoprotein, Osteogenic differentiation, Peptide amphiphile
Citation
Published Version (Please cite this version)