Bioinspired hydrogel surfaces to augment corneal endothelial cell monolayer formation

Simple item page
buir.contributor.authorElbuken, Çağlar
buir.contributor.orcidElbuken, Çağlar|0000-0001-8359-6871
dc.citation.epage255en_US
dc.citation.spage244en_US
dc.citation.volumeNumber15en_US
dc.contributor.authorElbuken, Çağlar
dc.date.accessioned2022-04-26T10:05:17Z
dc.date.available2022-04-26T10:05:17Z
dc.date.issued2020-12-26
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractCorneal endothelial cells (CECs) have limited proliferation ability leading to corneal endothelium (CE) dysfunction and eventually vision loss when cell number decreases below a critical level. Although transplantation is the main treatment method, donor shortage problem is a major bottleneck. The transplantation of in vitro developed endothelial cells with desirable density is a promising idea. Designing cell substrates that mimic the native CE microenvironment is a substantial step to achieve this goal. In the presented study, we prepared polyacrylamide (PA) cell substrates that have a microfabricated topography inspired by the dimensions of CECs. Hydrogel surfaces were prepared via two different designs with small and large patterns. Small patterned hydrogels have physiologically relevant hexagon densities (∼2000 hexagons/mm2), whereas large patterned hydrogels have sparsely populated hexagons (∼400 hexagons/mm2). These substrates have similar elastic modulus of native Descemet's membrane (DM; ∼50 kPa) and were modified with Collagen IV (Col IV) to have biochemical content similar to native DM. The behavior of bovine corneal endothelial cells on these substrates was investigated and results show that cell proliferation on small patterned substrates was significantly (p ¼ 0.0004) higher than the large patterned substrates. Small patterned substrates enabled a more densely populated cell monolayer compared to other groups (p ¼ 0.001 vs. flat and p < 0.0001 vs. large patterned substrates). These results suggest that generating bioinspired surface topographies augments the formation of CE monolayers with the desired cell density, addressing the in vitro development of CE layers.en_US
dc.description.provenanceSubmitted by Cem Çağatay Akgün (cem.akgun@bilkent.edu.tr) on 2022-04-26T10:05:17Z No. of bitstreams: 1 Bioinspired_hydrogel_surfaces_to_augment_corneal_endothelial_cell_monolayer_formation.pdf: 1941425 bytes, checksum: b222b25c75d99842671ced6ea648be90 (MD5)en
dc.description.provenanceMade available in DSpace on 2022-04-26T10:05:17Z (GMT). No. of bitstreams: 1 Bioinspired_hydrogel_surfaces_to_augment_corneal_endothelial_cell_monolayer_formation.pdf: 1941425 bytes, checksum: b222b25c75d99842671ced6ea648be90 (MD5) Previous issue date: 2020-12-26en
dc.identifier.doi10.1002/term.3173en_US
dc.identifier.eissn1932-7005
dc.identifier.issn1932-6254
dc.identifier.urihttp://hdl.handle.net/11693/78155
dc.language.isoEnglishen_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.relation.isversionofhttps://dx.doi.org/10.1002/term.3173en_US
dc.source.titleJ of Tissue Engineering and Regenerative Medicineen_US
dc.subjectBioinspirationen_US
dc.subjectBovine corneal endothelial cellsen_US
dc.subjectCorneal endotheliumen_US
dc.subjectCorneal regenerationen_US
dc.subjectPolyacrylamideen_US
dc.subjectSubstrate topographyen_US
dc.titleBioinspired hydrogel surfaces to augment corneal endothelial cell monolayer formationen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Bioinspired_hydrogel_surfaces_to_augment_corneal_endothelial_cell_monolayer_formation.pdf
Size:
1.85 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.69 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Institute of Materials Science and Nanotechnology (UNAM)