Toxicity of internalized laser generated pure silver nanoparticles to the isolated rat hippocampus cells

dc.citation.epage563en_US
dc.citation.issueNumber7en_US
dc.citation.spage555en_US
dc.citation.volumeNumber33en_US
dc.contributor.authorKursungoz, C.en_US
dc.contributor.authorTaş, S. T.en_US
dc.contributor.authorSargon, M. F.en_US
dc.contributor.authorSara, Y.en_US
dc.contributor.authorOrtaç, B.en_US
dc.date.accessioned2018-04-12T11:01:30Z
dc.date.available2018-04-12T11:01:30Z
dc.date.issued2017-02en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractSilver nanoparticles (AgNPs) are the most commonly used nanoparticles (NPs) in medicine, industry and cosmetics. They are generally considered as biocompatible. However, contradictory reports on their biosafety render them difficult to accept as 'safe'. In this study, we evaluated the neurotoxicity of direct AgNP treatment in rat hippocampal slices. We produced pure uncoated AgNPs by a pulsed laser ablation method. NP characterization was performed by Ultraviolet (UV) visible spectrophotometer, scanning electron microscope, transmission electron microscope (TEM) and energy-dispersive X-ray spectroscopy. Rat hippocampal slices were treated with AgNPs for an hour. AgNP exposure of hippocampal tissue resulted in a significant decrease in cell survival in a dose-dependent manner. Our TEM results showed that AgNPs were distributed in the extracellular matrix and were taken into the cytoplasm of the neurons. Moreover, we found that only larger AgNPs were taken into the neurons via phagocytosis. This study showed that the pure AgNPs produced by laser ablation are toxic to the neural tissue. We also found that neurons internalized only the large NPs by phagocytosis which seems to be the major mechanism in AgNP neurotoxicity.en_US
dc.identifier.doi10.1177/0748233717690992en_US
dc.identifier.issn0748-2337
dc.identifier.urihttp://hdl.handle.net/11693/37056
dc.language.isoEnglishen_US
dc.publisherSAGEen_US
dc.relation.isversionofhttps://doi.org/10.1177/0748233717690992en_US
dc.source.titleToxicology and Industrial Healthen_US
dc.subjectClassical endocytosisen_US
dc.subjectHippocampusen_US
dc.subjectInternalization of nanoparticlesen_US
dc.subjectLaser ablationen_US
dc.subjectNeurodegenerative diseaseen_US
dc.subjectSilver nanoparticlesen_US
dc.subjectSilver nanoparticleen_US
dc.subjectAnimal tissueen_US
dc.subjectArticleen_US
dc.subjectBrain nerve cellen_US
dc.subjectCell survivalen_US
dc.subjectCell viabilityen_US
dc.subjectControlled studyen_US
dc.subjectCytoplasmen_US
dc.subjectCytotoxicityen_US
dc.subjectElectron microscopeen_US
dc.subjectEnergy dispersive X ray spectroscopyen_US
dc.subjectExtracellular matrixen_US
dc.subjectHippocampal sliceen_US
dc.subjectHippocampal tissueen_US
dc.subjectHippocampusen_US
dc.subjectMaleen_US
dc.subjectNanotoxicologyen_US
dc.subjectNeodymium YLF laseren_US
dc.subjectNeurotoxicityen_US
dc.subjectNonhumanen_US
dc.subjectPhagocytosisen_US
dc.subjectRaten_US
dc.subjectScanning electron microscopeen_US
dc.subjectScanning electron microscopyen_US
dc.subjectTransmission electron microscopyen_US
dc.subjectUltraviolet spectrophotometeren_US
dc.subjectUltraviolet spectrophotometryen_US
dc.titleToxicity of internalized laser generated pure silver nanoparticles to the isolated rat hippocampus cellsen_US
dc.typeArticleen_US
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