Laminin mimetic peptide nanofibers regenerate acute muscle defect

dc.citation.epage200en_US
dc.citation.spage190en_US
dc.citation.volumeNumber60en_US
dc.contributor.authorCimenci, C. E.en_US
dc.contributor.authorUzunalli, G.en_US
dc.contributor.authorUysal, O.en_US
dc.contributor.authorYergoz, F.en_US
dc.contributor.authorUmay, E. K.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.contributor.bilkentauthorGüler, Mustafa O.
dc.date.accessioned2018-04-12T11:13:33Z
dc.date.available2018-04-12T11:13:33Z
dc.date.issued2017en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractSkeletal muscle cells are terminally differentiated and require the activation of muscle progenitor (satellite) cells for their regeneration. There is a clinical need for faster and more efficient treatment methods for acute muscle injuries, and the stimulation of satellite cell proliferation is promising in this context. In this study, we designed and synthesized a laminin-mimetic bioactive peptide (LM/E-PA) system that is capable of accelerating satellite cell activation by emulating the structure and function of laminin, a major protein of the basal membrane of the skeletal muscle. The LM/E-PA nanofibers enhance myogenic differentiation in vitro and the clinical relevance of the laminin-mimetic bioactive scaffold system was demonstrated further by assessing its effect on the regeneration of acute muscle injury in a rat model. Laminin mimetic peptide nanofibers significantly promoted satellite cell activation in skeletal muscle and accelerated myofibrillar regeneration following acute muscle injury. In addition, the LM/E-PA scaffold treatment significantly reduced the time required for the structural and functional repair of skeletal muscle. This study represents one of the first examples of molecular- and tissue-level regeneration of skeletal muscle facilitated by bioactive peptide nanofibers following acute muscle injury. Significance Statement Sports, heavy lifting and other strength-intensive tasks are ubiquitous in modern life and likely to cause acute skeletal muscle injury. Speeding up regeneration of skeletal muscle injuries would not only shorten the duration of recovery for the patient, but also support the general health and functionality of the repaired muscle tissue. In this work, we designed and synthesized a laminin-mimetic nanosystem to enhance muscle regeneration. We tested its activity in a rat tibialis anterior muscle by injecting the bioactive nanosystem. The evaluation of the regeneration and differentiation capacity of skeletal muscle suggested that the laminin-mimetic nanosystem enhances skeletal muscle regeneration and provides a suitable platform that is highly promising for the regeneration of acute muscle injuries. This work demonstrates for the first time that laminin-mimetic self-assembled peptide nanosystems facilitate myogenic differentiation in vivo without the need for additional treatment.en_US
dc.identifier.doi10.1016/j.actbio.2017.07.010en_US
dc.identifier.eissn1878-7568en_US
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/11693/37442
dc.language.isoEnglishen_US
dc.publisherActa Materialia Incen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.actbio.2017.07.010en_US
dc.source.titleActa Biomaterialiaen_US
dc.subjectAcute muscle injuryen_US
dc.subjectFunctional self-assemblyen_US
dc.subjectHydrogelen_US
dc.subjectLamininen_US
dc.subjectPeptide nanofiberen_US
dc.subjectRegenerationen_US
dc.subjectCardiotoxinen_US
dc.subjectLamininen_US
dc.subjectMyoD1 proteinen_US
dc.subjectMyogeninen_US
dc.subjectNanofiberen_US
dc.subjectPeptideen_US
dc.subjectTranscription factor PAX7en_US
dc.subjectAnimal behavioren_US
dc.subjectAnimal experimenten_US
dc.subjectAnimal modelen_US
dc.subjectAnimal tissueen_US
dc.subjectArticleen_US
dc.subjectBasal laminaen_US
dc.subjectCell activationen_US
dc.subjectCell differentiationen_US
dc.subjectCircular dichroismen_US
dc.subjectComparative studyen_US
dc.subjectControlled studyen_US
dc.subjectFlow kineticsen_US
dc.subjectHigh performance liquid chromatographyen_US
dc.subjectHydrogelen_US
dc.subjectIn vitro studyen_US
dc.subjectIn vivo studyen_US
dc.subjectLiquid chromatography-mass spectrometryen_US
dc.subjectMaleen_US
dc.subjectMuscle cellen_US
dc.subjectMuscle developmenten_US
dc.subjectMuscle diseaseen_US
dc.subjectMuscle regenerationen_US
dc.subjectNonhumanen_US
dc.subjectpHen_US
dc.subjectPriority journalen_US
dc.subjectProtein analysisen_US
dc.subjectProtein expressionen_US
dc.subjectProtein functionen_US
dc.subjectProtein structureen_US
dc.subjectRaten_US
dc.subjectReverse transcription polymerase chain reactionen_US
dc.subjectSatellite cellen_US
dc.subjectScanning electron microscopyen_US
dc.subjectSynthesisen_US
dc.subjectTransmission electron microscopyen_US
dc.subjectWestern blottingen_US
dc.titleLaminin mimetic peptide nanofibers regenerate acute muscle defecten_US
dc.typeArticleen_US
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