Biocatalytic protein membranes fabricated by electrospinning

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dc.citation.epage32en_US
dc.citation.spage26en_US
dc.citation.volumeNumber103en_US
dc.contributor.authorKabay, G.en_US
dc.contributor.authorKaleli, G.en_US
dc.contributor.authorSultanova, Z.en_US
dc.contributor.authorÖlmez, T. T.en_US
dc.contributor.authorŞeker, U. Ö. Ş.en_US
dc.contributor.authorMutlu, M.en_US
dc.date.accessioned2018-04-12T10:53:04Z
dc.date.available2018-04-12T10:53:04Z
dc.date.issued2016en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractIn this study, a protein-based catalytic membrane was produced by electrospinning. Membrane activity was characterised in terms of response current for various glucose concentrations. We focused on the preparation of a scaffold by converting a globular protein to other structural forms using catastrophic solvents. A scaffolding protein, bovine serum albumin, and an enzyme, glucose oxidase (GOD), were selected as a model natural carrier matrix and a biologically active agent, respectively. Beta-mercaptoethanol (β-ME) was used to convert the globular protein to an amyloid-like form. A structural stabilising agent, 2,2,2-triflouroethanol (TFE), was used to maintain the final α-helical structure of the amyloid-like protein. The TFE:PBS (phosphate-buffered saline) ratio and various electrospinning parameters were analysed to minimise activity loss. Using this approach, we applied electrospinning to an active enzyme to obtain biocatalytic nanofibrous membranes. After optimising the protein electrospinning process, the activities of the protein nanofibrous membranes were monitored. GOD remained active in the new membrane structure. The highest enzyme activity was observed for the membranes prepared with a 1.5:1 (v:v) TFE:PBS solvent ratio. In that particular case, the immobilized enzyme created a current of 0.7 μA and the apparent activity was 2547 ± 132 U/m2.en_US
dc.description.provenanceMade available in DSpace on 2018-04-12T10:53:04Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2016en
dc.identifier.doi10.1016/j.reactfunctpolym.2016.03.015en_US
dc.identifier.issn1381-5148
dc.identifier.urihttp://hdl.handle.net/11693/36780
dc.language.isoEnglishen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionofhttps://doi.org/10.1016/j.reactfunctpolym.2016.03.015en_US
dc.source.titleReactive and Functional Polymersen_US
dc.subjectAmperometric detectionen_US
dc.subjectBiocatalytic membraneen_US
dc.subjectBovine serum albuminen_US
dc.subjectElectrospinningen_US
dc.subjectGlucose oxidaseen_US
dc.subjectBody fluidsen_US
dc.subjectElectrospinningen_US
dc.subjectEnzyme activityen_US
dc.subjectEnzymesen_US
dc.subjectGlucoseen_US
dc.subjectGlucose oxidaseen_US
dc.subjectGlucose sensorsen_US
dc.subjectGlycoproteinsen_US
dc.subjectMammalsen_US
dc.subjectMembranesen_US
dc.subjectNanofibersen_US
dc.subjectProteinsen_US
dc.subjectSpinning (fibers)en_US
dc.subjectSurface plasmon resonanceen_US
dc.subjectAmperometric detectionen_US
dc.subjectBiocatalytic membranesen_US
dc.subjectBovine serum albuminsen_US
dc.subjectElectrospinning parametersen_US
dc.subjectElectrospinning processen_US
dc.subjectGlucose concentrationen_US
dc.subjectNanofibrous membranesen_US
dc.subjectPhosphate-buffered salinesen_US
dc.subjectScaffolds (biology)en_US
dc.titleBiocatalytic protein membranes fabricated by electrospinningen_US
dc.typeArticleen_US

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