Real-time and selective detection of single nucleotide DNA mutations using surface engineered microtoroids

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buir.contributor.authorBayındır, Mehmet
dc.citation.epage10926en_US
dc.citation.issueNumber21en_US
dc.citation.spage10920en_US
dc.citation.volumeNumber87en_US
dc.contributor.authorToren, P.en_US
dc.contributor.authorOzgur E.en_US
dc.contributor.authorBayındır, Mehmeten_US
dc.date.accessioned2016-02-08T11:01:18Z
dc.date.available2016-02-08T11:01:18Z
dc.date.issued2015en_US
dc.departmentDepartment of Physicsen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractMictoroids, as optical biosensors, can provide beneficial biosensing platforms to understand DNA alterations. These alterations could have significant clinical importance, such as the case of Pseudomonas aeruginosa, which is a commonly found pathogen in Cystic Fibrosis (CF) patients-causing poor prognosis by undergoing mutations during disease steps, gaining virulence and drug resistance. To provide a preliminary diagnosis platform for early-stage bacterial mutations, biosensing with a selective microtoroid surface was suggested. For this purpose, microtoroids with high quality factors were fabricated. The microtoroid surfaces were coated with (3-aminopropyl) triethoxysilane (APTES)/trimethylmethoxysilane (TMMS) mixed silane solution followed by EDC/NHS chemistry for covalent conjugation of DNA probes. Ethanolamine capping was applied to avoid unspecific interactions. The confocal studies confirmed homogeneous functionalization of the microtoroid surface. The DNA hybridization was demonstrated to be affected from the probe length. The optical biosensors showed a significant response (∼22 pm) to the complementary strand of the mutated type P. aeruginosa DNA, while showing substantially low and late response (∼5 pm) to the point mismatch strand. The limit of detection (LOD) for the complementary strand was calculated as 2.32 nM. No significant response was obtained for the noncomplementary strand. The results showed the microtoroids possessed selective surfaces in terms of distinguishing DNA alterations.en_US
dc.identifier.doi10.1021/acs.analchem.5b02664en_US
dc.identifier.issn0003-2700
dc.identifier.urihttp://hdl.handle.net/11693/26541
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/acs.analchem.5b02664en_US
dc.source.titleAnalytical Chemistryen_US
dc.subjectBiosensorsen_US
dc.subjectDiagnosisen_US
dc.subjectDrug therapyen_US
dc.subjectSilanesen_US
dc.subjectBiosensing platformsen_US
dc.subjectComplementary stranden_US
dc.subjectFunctionalizationsen_US
dc.subjectHigh quality factorsen_US
dc.subjectOptical bio-sensorsen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectSelective detectionen_US
dc.subjectSingle nucleotidesen_US
dc.subjectDNAen_US
dc.titleReal-time and selective detection of single nucleotide DNA mutations using surface engineered microtoroidsen_US
dc.typeArticleen_US

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Department of Physics
Institute of Materials Science and Nanotechnology (UNAM)
Nanotechnology Research Center (NANOTAM)