Three-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation
buir.contributor.author | Gunay, Gokhan | |
buir.contributor.author | Sever, Melike | |
buir.contributor.author | Tekinay, Ayse B. | |
buir.contributor.author | Güler, Mustafa O. | |
dc.citation.epage | 9 | en_US |
dc.citation.issueNumber | 12 | en_US |
dc.citation.spage | 1 | en_US |
dc.citation.volumeNumber | 12 | en_US |
dc.contributor.author | Gunay, Gokhan | en_US |
dc.contributor.author | Sever, Melike | en_US |
dc.contributor.author | Tekinay, Ayse B. | en_US |
dc.contributor.author | Güler, Mustafa O. | en_US |
dc.date.accessioned | 2018-04-12T10:39:07Z | |
dc.date.available | 2018-04-12T10:39:07Z | |
dc.date.issued | 2017 | en_US |
dc.department | Institute of Materials Science and Nanotechnology (UNAM) | en_US |
dc.department | Interdisciplinary Program in Neuroscience (NEUROSCIENCE) | en_US |
dc.department | Aysel Sabuncu Brain Research Center (BAM) | en_US |
dc.description.abstract | The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein-derived epitope in two-dimensional (2D) and three-dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC-12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | en_US |
dc.description.provenance | Made available in DSpace on 2018-04-12T10:39:07Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2017 | en |
dc.embargo.release | 2018-12-18 | en_US |
dc.identifier.doi | 10.1002/biot.201700080 | en_US |
dc.identifier.issn | 1860-6768 | |
dc.identifier.uri | http://hdl.handle.net/11693/36417 | |
dc.language.iso | English | en_US |
dc.publisher | Wiley-VCH Verlag | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1002/biot.201700080 | en_US |
dc.source.title | Biotechnology Journal | en_US |
dc.subject | 2D and 3D cell culture | en_US |
dc.subject | Extracellular matrix | en_US |
dc.subject | Laminin | en_US |
dc.subject | Bioactivity | en_US |
dc.subject | Biomimetics | en_US |
dc.subject | Cell culture | en_US |
dc.subject | Cells | en_US |
dc.subject | Cytology | en_US |
dc.subject | Gels | en_US |
dc.subject | Peptides | en_US |
dc.subject | 3-D cell culture | en_US |
dc.subject | Extracellular matrices | en_US |
dc.subject | Laminin | en_US |
dc.subject | Neural differentiations | en_US |
dc.subject | Peptide amphiphiles | en_US |
dc.subject | Scaffolds (biology) | en_US |
dc.subject | Amphophile | en_US |
dc.subject | Beta III tubulin | en_US |
dc.subject | Beta tubulin | en_US |
dc.subject | Epitope | en_US |
dc.subject | Nanofiber | en_US |
dc.subject | Nerve growth factor | en_US |
dc.subject | Peptidomimetic agent | en_US |
dc.subject | Unclassified drug | en_US |
dc.subject | Controlled study | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Hydrogel | en_US |
dc.subject | Nerve cell differentiation | en_US |
dc.subject | Neurite | en_US |
dc.subject | PC12 cell line | en_US |
dc.subject | Priority journal | en_US |
dc.subject | Protein expression | en_US |
dc.title | Three-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation | en_US |
dc.type | Article | en_US |
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