Three-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

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buir.contributor.authorGunay, Gokhan
buir.contributor.authorSever, Melike
buir.contributor.authorTekinay, Ayse B.
buir.contributor.authorGüler, Mustafa O.
dc.citation.epage9en_US
dc.citation.issueNumber12en_US
dc.citation.spage1en_US
dc.citation.volumeNumber12en_US
dc.contributor.authorGunay, Gokhanen_US
dc.contributor.authorSever, Melikeen_US
dc.contributor.authorTekinay, Ayse B.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.date.accessioned2018-04-12T10:39:07Z
dc.date.available2018-04-12T10:39:07Z
dc.date.issued2017en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.description.abstractThe extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein-derived epitope in two-dimensional (2D) and three-dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC-12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.embargo.release2018-12-18en_US
dc.identifier.doi10.1002/biot.201700080en_US
dc.identifier.issn1860-6768
dc.identifier.urihttp://hdl.handle.net/11693/36417
dc.language.isoEnglishen_US
dc.publisherWiley-VCH Verlagen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/biot.201700080en_US
dc.source.titleBiotechnology Journalen_US
dc.subject2D and 3D cell cultureen_US
dc.subjectExtracellular matrixen_US
dc.subjectLamininen_US
dc.subjectBioactivityen_US
dc.subjectBiomimeticsen_US
dc.subjectCell cultureen_US
dc.subjectCellsen_US
dc.subjectCytologyen_US
dc.subjectGelsen_US
dc.subjectPeptidesen_US
dc.subject3-D cell cultureen_US
dc.subjectExtracellular matricesen_US
dc.subjectLamininen_US
dc.subjectNeural differentiationsen_US
dc.subjectPeptide amphiphilesen_US
dc.subjectScaffolds (biology)en_US
dc.subjectAmphophileen_US
dc.subjectBeta III tubulinen_US
dc.subjectBeta tubulinen_US
dc.subjectEpitopeen_US
dc.subjectNanofiberen_US
dc.subjectNerve growth factoren_US
dc.subjectPeptidomimetic agenten_US
dc.subjectUnclassified drugen_US
dc.subjectControlled studyen_US
dc.subjectGene expressionen_US
dc.subjectHydrogelen_US
dc.subjectNerve cell differentiationen_US
dc.subjectNeuriteen_US
dc.subjectPC12 cell lineen_US
dc.subjectPriority journalen_US
dc.subjectProtein expressionen_US
dc.titleThree-Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiationen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)
Aysel Sabuncu Brain Research Center (BAM)