Label-free nanometer-resolution imaging of biological architectures through surface enhanced raman scattering

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buir.contributor.authorAyas, Sencer
buir.contributor.authorÇınar, Göksu
buir.contributor.authorÖzkan, Alper Devrim
buir.contributor.authorSoran, Zeliha
buir.contributor.authorEkiz, Oner
buir.contributor.authorKocaay, Deniz
buir.contributor.authorToren, Pelin
buir.contributor.authorKaya, Yasin
buir.contributor.authorTekinay, Ayse Begum
buir.contributor.authorGüler, Mustafa O.
buir.contributor.authorDana, Aykutlu
dc.citation.epage2624en_US
dc.citation.spage2624en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorAyas, Senceren_US
dc.contributor.authorÇınar, Göksuen_US
dc.contributor.authorÖzkan, Alper Devrimen_US
dc.contributor.authorSoran, Zelihaen_US
dc.contributor.authorEkiz, Oneren_US
dc.contributor.authorKocaay, Denizen_US
dc.contributor.authorTomak, A.en_US
dc.contributor.authorToren, Pelinen_US
dc.contributor.authorKaya, Yasinen_US
dc.contributor.authorTunc, I.en_US
dc.contributor.authorZareie, H.en_US
dc.contributor.authorTekinay, T.en_US
dc.contributor.authorTekinay, Ayse Begumen_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorDana, Aykutluen_US
dc.date.accessioned2015-07-28T12:00:24Z
dc.date.available2015-07-28T12:00:24Z
dc.date.issued2013en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentDepartment of Electrical and Electronics Engineeringen_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.description.abstractLabel free imaging of the chemical environment of biological specimens would readily bridge the supramolecular and the cellular scales, if a chemical fingerprint technique such as Raman scattering can be coupled with super resolution imaging. We demonstrate the possibility of label-free super-resolution Raman imaging, by applying stochastic reconstruction to temporal fluctuations of the surface enhanced Raman scattering (SERS) signal which originate from biomolecular layers on large-area plasmonic surfaces with a high and uniform hot-spot density (> 10(11)/cm(2), 20 to 35 nm spacing). A resolution of 20 nm is demonstrated in reconstructed images of self-assembled peptide network and fibrilated lamellipodia of cardiomyocytes. Blink rate density is observed to be proportional to the excitation intensity and at high excitation densities (> 10 kW/cm(2)) blinking is accompanied by molecular breakdown. However, at low powers, simultaneous Raman measurements show that SERS can provide sufficient blink rates required for image reconstruction without completely damaging the chemical structure.en_US
dc.description.provenanceMade available in DSpace on 2015-07-28T12:00:24Z (GMT). No. of bitstreams: 1 10.1038-srep02624.pdf: 1277887 bytes, checksum: 594185753f52975856646f277589cd3a (MD5)en
dc.identifier.doi10.1038/srep02624en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11693/12174
dc.language.isoEnglishen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep02624en_US
dc.source.titleScientific Reportsen_US
dc.titleLabel-free nanometer-resolution imaging of biological architectures through surface enhanced raman scatteringen_US
dc.typeArticleen_US

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