Comparison of Au(III) and Ga(III) ions' binding to calf thymus DNA: Spectroscopic characterization and thermal analysis

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dc.citation.epage452en_US
dc.citation.issueNumber3en_US
dc.citation.spage445en_US
dc.citation.volumeNumber160en_US
dc.contributor.authorSarioglu O.F.en_US
dc.contributor.authorTekiner-Gursacli, R.en_US
dc.contributor.authorOzdemir, A.en_US
dc.contributor.authorTekinay, T.en_US
dc.date.accessioned2016-02-08T11:01:08Z
dc.date.available2016-02-08T11:01:08Z
dc.date.issued2014en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractMetals have been studied as potential chemotherapeutic agents for cancer therapies due to their high reactivity toward a wide variety of substances. The characterization of metal ion-binding capacities is essential to understand the possible effects of metals on target biomolecules. In the present study, biochemical effects of Au(III) and Ga(III) ions on calf thymus DNA (ctDNA) were studied comparatively via bioanalytical, spectroscopic, and thermal methods. Briefly, UV-Vis absorbance spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and Fourier transform infrared (FT-IR) spectroscopy were utilized for spectroscopic characterization, and isothermal titration calorimetry (ITC) measurements were performed for thermal analysis. Our results reveal that both Au(III) and Ga(III) ions are capable of interacting with ctDNA, and Au(III) ions display a more favorable interaction and a higher binding affinity. ITC analyses indicate that the Au(III)-DNA interaction displays a binding affinity (Ka) around 1.43×106 M -1, while a Ka around 1.17×105 M -1 was observed for the Ga(III)-DNA binding. It was suggested that both metal ions are unlikely to change the structural B-conformation while interacting with ctDNA. © 2014 Springer Science+Business Media.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T11:01:08Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1007/s12011-014-0059-8en_US
dc.identifier.issn0163-4984
dc.identifier.urihttp://hdl.handle.net/11693/26529
dc.language.isoEnglishen_US
dc.publisherHumana Press Inc.en_US
dc.publisherSpringeren_US
dc.relation.isversionofhttps://doi.org/10.1007/s12011-014-0059-8en_US
dc.source.titleBiological Trace Element Researchen_US
dc.subjectAu(III) and Ga(III) ionsen_US
dc.subjectB-conformation of DNAen_US
dc.subjectCalf thymus DNAen_US
dc.subjectDNA-binding studiesen_US
dc.subjectDNAen_US
dc.subjectGalliumen_US
dc.subjectGolden_US
dc.subjectAnimalsen_US
dc.subjectCattleen_US
dc.subjectDNAen_US
dc.subjectGalliumen_US
dc.subjectGolden_US
dc.titleComparison of Au(III) and Ga(III) ions' binding to calf thymus DNA: Spectroscopic characterization and thermal analysisen_US
dc.typeArticleen_US

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