Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering

Date
2012
Authors
Soran, Z.
Aydin, R.S.T.
Gumusderelioglu, M.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Journal of Microencapsulation
Print ISSN
0265-2048
Electronic ISSN
Publisher
Volume
29
Issue
8
Pages
770 - 780
Language
English
Type
Article
Journal Title
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Volume Title
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Abstract

The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50ng or 100ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation. © 2012 Informa UK Ltd All rights reserved.

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Keywords
Alginate microspheres, BMP-6, Chitosan scaffold, Mesenchymal stem cells, Periodontal tissue engineering, alginic acid, bone morphogenetic protein 6, chitosan, microsphere, animal cell, article, bone marrow, cell culture, cell differentiation, controlled release formulation, culture medium, drug release, electrospray, mesenchymal stem cell, nonhuman, periodontium, rat, tissue engineering, Alginates, Animals, Bone Morphogenetic Protein 6, Chitosan, Culture Media, Glucuronic Acid, Hexuronic Acids, Mesenchymal Stromal Cells, Microscopy, Electron, Scanning, Microspheres, Periodontium, Rats, Tissue Engineering
Citation
Published Version (Please cite this version)