Electrostatic effects on nanofiber formation of self-assembling peptide amphiphiles

Date
2011
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Source Title
Journal of Colloid and Interface Science
Print ISSN
0021-9797
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Publisher
Elsevier
Volume
356
Issue
1
Pages
131 - 137
Language
English
Type
Article
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Abstract

Self-assembling peptide amphiphile molecules have been of interest to various tissue engineering studies. These molecules self-assemble into nanofibers which organize into three-dimensional networks to form hydrocolloid systems mimicking the extracellular matrix. The formation of nanofibers is affected by the electrostatic interactions among the peptides. In this work, we studied the effect of charged groups on the peptides on nanofiber formation. The self-assembly process was studied by pH and zeta potential measurements, FT-IR, circular dichroism, rheology, atomic force microscopy, scanning electron microscopy and transmission electron microscopy. The aggregation of the peptides was triggered upon neutralization of the charged residues by pH change or addition of electrolyte or biomacromolecules. Understanding the controlled formation of the hydrocolloid gels composed of peptide amphiphile nanofibers can lead us to develop in situ gel forming bioactive collagen mimetic nanofibers for various tissue engineering studies including bioactive surface coatings. © 2010 Elsevier Inc.

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Keywords
Electrostatic interactions, Gel, Nanofiber, Peptide, Peptide amphiphile, Self-assembly, Bioactive surfaces, Biomacromolecules, Charged groups, Charged residues, Circular dichroism, Electrostatic effect, Electrostatic interactions, Extracellular matrices, In-situ, Peptide amphiphiles, pH change, Self-assemble, Three-dimensional networks, Zeta potential measurements, Atomic force microscopy, Biomimetics, Coatings, Dichroism, Electrostatics, Tissue engineering, Transmission electron microscopy, Zeta potential, Hydrogen-Ion Concentration
Citation
Published Version (Please cite this version)