4,5-dianilinophtalimide protects neuroendocrine cells against serum deprivation-induced stress and apoptosis

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4,5-dianilinophtalimide protects neuroendocrine cells against serum deprivation-induced stress and apoptosis.pdf (1.72 MB)

Date

2013

Authors

Ergin V.
Erdogan, M.
Karasu Ç.
Menevşe, A.

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Source Title

Neuroendocrinology Letters

Print ISSN

0172780X

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Volume

34

Issue

5

Pages

359 - 365

Language

English

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Article

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Abstract

OBJECTIVE: The aim of this study was to reveal the effects of 4,5-dianilinophthalimide (DAPH), which inhibits amyloid β fibrillization, against serum deprivation (SD)-induced apoptosis and the possible mechanisms in differentiated PC12 neuron cells. METHODS: Firstly, we evaluated whether DAPH protects cell viability exposed to SD by MTT assay. Next, we examined the changes of phospho-p38 MAPK (Thr180/Tyr182), phospho-HSP27 (Ser82), phospho-c-JUN (Ser73) and cleaved-CASP3 (Asp175) profiles by immunoblotting, in PC12 cells exposed to SD. Intracellular reactive oxygen species (ROS) level was also measured. RESULTS: SD induced apoptosis accompanied by up-regulation of phospho-p38 MAPK (Thr180/Tyr182), phospho-HSP27 (Ser82), phospho-c-JUN (Ser73), cleaved-CASP3 (Asp175) and intracellular ROS content. Co-treatment with nontoxic doses of DAPH prevented apoptosis by the attenuation of activated proteins and reduction of ROS level. These results suggest that serum deprivation-induced apoptosis inhibited by DAPH administration. CONCLUSION: We have provided for the first evidence that DAPH has a neuroprotective effect on SD-caused stress, probably via contributing the reestablishment of redox homeostasis. © 2013 Neuroendocrinology Letters.

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Keywords

Apoptosis , Cellular stress , DAPH , PC12 , Serum deprivation , 4,5 dianilinophtalimide , caspase 3 , heat shock protein 27 , mitogen activated protein kinase p38 , neuroprotective agent , protein c jun , reactive oxygen metabolite , unclassified drug , apoptosis , article , attenuation , cell viability , drug effect , immunoblotting , neuroprotection , neurosecretory cell , oxidative stress , PC12 cell , serum deprivation induced apoptosis , serum deprivation induced stress , upregulation , Animals , Apoptosis , Caspase 3 , Cell Survival , HSP27 Heat-Shock Proteins , Neurons , Neuroprotective Agents , p38 Mitogen-Activated Protein Kinases , PC12 Cells , Phosphorylation , Phthalimides , Proto-Oncogene Proteins c-jun , Rats , Reactive Oxygen Species , Stress, Physiological , Up-Regulation

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http://hdl.handle.net/11693/20765

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Institute of Materials Science and Nanotechnology (UNAM)