4,5-dianilinophtalimide protects neuroendocrine cells against serum deprivation-induced stress and apoptosis

Date
2013
Authors
Ergin V.
Erdogan, M.
Karasu Ç.
Menevşe, A.
Advisor
Instructor
Source Title
Neuroendocrinology Letters
Print ISSN
0172780X
Electronic ISSN
Publisher
Volume
34
Issue
5
Pages
359 - 365
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Abstract

OBJECTIVE: The aim of this study was to reveal the effects of 4,5-dianilinophthalimide (DAPH), which inhibits amyloid β fibrillization, against serum deprivation (SD)-induced apoptosis and the possible mechanisms in differentiated PC12 neuron cells. METHODS: Firstly, we evaluated whether DAPH protects cell viability exposed to SD by MTT assay. Next, we examined the changes of phospho-p38 MAPK (Thr180/Tyr182), phospho-HSP27 (Ser82), phospho-c-JUN (Ser73) and cleaved-CASP3 (Asp175) profiles by immunoblotting, in PC12 cells exposed to SD. Intracellular reactive oxygen species (ROS) level was also measured. RESULTS: SD induced apoptosis accompanied by up-regulation of phospho-p38 MAPK (Thr180/Tyr182), phospho-HSP27 (Ser82), phospho-c-JUN (Ser73), cleaved-CASP3 (Asp175) and intracellular ROS content. Co-treatment with nontoxic doses of DAPH prevented apoptosis by the attenuation of activated proteins and reduction of ROS level. These results suggest that serum deprivation-induced apoptosis inhibited by DAPH administration. CONCLUSION: We have provided for the first evidence that DAPH has a neuroprotective effect on SD-caused stress, probably via contributing the reestablishment of redox homeostasis. © 2013 Neuroendocrinology Letters.

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Keywords
Apoptosis, Cellular stress, DAPH, PC12, Serum deprivation, 4,5 dianilinophtalimide, caspase 3, heat shock protein 27, mitogen activated protein kinase p38, neuroprotective agent, protein c jun, reactive oxygen metabolite, unclassified drug, apoptosis, article, attenuation, cell viability, drug effect, immunoblotting, neuroprotection, neurosecretory cell, oxidative stress, PC12 cell, serum deprivation induced apoptosis, serum deprivation induced stress, upregulation, Animals, Apoptosis, Caspase 3, Cell Survival, HSP27 Heat-Shock Proteins, Neurons, Neuroprotective Agents, p38 Mitogen-Activated Protein Kinases, PC12 Cells, Phosphorylation, Phthalimides, Proto-Oncogene Proteins c-jun, Rats, Reactive Oxygen Species, Stress, Physiological, Up-Regulation
Citation
Published Version (Please cite this version)