The role of bcsE gene in the pathogenicity of Salmonella

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buir.contributor.authorÖzdemir, Caner
buir.contributor.authorEvcili, İrem
buir.contributor.authorKahraman, Tamer
buir.contributor.authorGürsel, İhsan
buir.contributor.orcidÖzdemir, Caner|https://orcid.org/0000-0003-1259-1824
buir.contributor.orcidEvcili, İrem|https://orcid.org/0000-0003-1259-1824
buir.contributor.orcidKahraman, Tamer|https://orcid.org/0000-0002-5527-7336
buir.contributor.orcidGürsel, İhsan|https://orcid.org/0000-0003-3761-1166
dc.citation.epage11en_US
dc.citation.issueNumber6en_US
dc.citation.spage1en_US
dc.citation.volumeNumber79en_US
dc.contributor.authorÖzdemir, Caner
dc.contributor.authorAkçelik, N.
dc.contributor.authorÖzdemir, F. N.
dc.contributor.authorEvcili, İrem
dc.contributor.authorKahraman, Tamer
dc.contributor.authorGürsel, İhsan
dc.contributor.authorAkçelik, M.
dc.date.accessioned2022-02-11T06:39:11Z
dc.date.available2022-02-11T06:39:11Z
dc.date.issued2021-07-19
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractThe effects of the bcsE gene and BcsE protein on bacterial physiology and pathogenicity in SalmonellaTyphimurium and Salmonella Group C1 were investigated. It was observed that biofilm and pellicle formation did not occur in the bcsE gene mutants of wild-type strains. Besides, the ‘rdar’ (red, dry, rough) biofilm morphotype in wild-type strains changed significantly in the mutants. In terms of the bcsE gene, the swimming and swarming motility in mutant strains showed a dramatic increase compared to the wild-type strains. The Salmonella bcsE gene was cloned into Escherichia coli BL21, and the his-tagged protein produced in this strain was purified to obtain polyclonal antibodies in BALB/c mice. The antibodies were showed labeled antigen specificity to the BscE protein. As a result of immunization and systemic persistence tests carried out with BALB/c mice, BscE protein was determined to trigger high levels of humoral and cellular responses (Th1 cytokine production, IgG2a/IgG1 > 1). Systemic persistence in the liver and spleen samples decreased by 99.99% and 100% in the bcsE mutant strains. Finally, invasion abilities on HT-29 epithelial cells of wild-type strains were utterly disappeared in their bcsE gene mutant strains.en_US
dc.identifier.doi10.1093/femspd/ftab037en_US
dc.identifier.eissn2049-632X
dc.identifier.urihttp://hdl.handle.net/11693/77258
dc.language.isoEnglishen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofhttps://doi.org/10.1093/femspd/ftab037en_US
dc.source.titlePathogens and Diseaseen_US
dc.subjectSalmonellaen_US
dc.subjectBcsEen_US
dc.subjectBiofilmen_US
dc.subjectInvasionen_US
dc.subjectPersistenceen_US
dc.subjectPathogenicityen_US
dc.titleThe role of bcsE gene in the pathogenicity of Salmonellaen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)
Department of Molecular Biology and Genetics