Colon cancer associated transcript-1 (CCAT1) expression in adenocarcinoma of the stomach

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dc.citation.epage110en_US
dc.citation.issueNumber2en_US
dc.citation.spage105en_US
dc.citation.volumeNumber6en_US
dc.contributor.authorMizrahi, I.en_US
dc.contributor.authorMazeh, H.en_US
dc.contributor.authorGrinbaum, R.en_US
dc.contributor.authorBeglaibter, N.en_US
dc.contributor.authorWilschanski, M.en_US
dc.contributor.authorPavlov, V.en_US
dc.contributor.authorAdileh, M.en_US
dc.contributor.authorStojadinovic, A.en_US
dc.contributor.authorAvital, I.en_US
dc.contributor.authorGure, A. O.en_US
dc.contributor.authorHalle, D.en_US
dc.contributor.authorNissan, A.en_US
dc.date.accessioned2016-02-08T10:13:19Z
dc.date.available2016-02-08T10:13:19Z
dc.date.issued2015en_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.description.abstractBackground: Long non-coding RNAs (lncRNAs) have been shown to have functional roles in cancer biology and are dys-regulated in many tumors. Colon Cancer Associated Transcript -1 (CCAT1) is a lncRNA, previously shown to be significantly up-regulated in colon cancer. The aim of this study is to determine expression levels of CCAT1 in gastric carcinoma (GC). Methods: Tissue samples were obtained from patients undergoing resection for gastric carcinoma (n=19). For each patient, tumor tissue and normal appearing gastric mucosa were taken. Normal gastric tissues obtained from morbidly obese patients, undergoing laparoscopic sleeve gastrectomy served as normal controls (n=19). A human gastric carcinoma cell line (AGS) served as positive control. RNA was extracted from all tissue samples and CCAT1 expression was analyzed using quantitative real time-PCR (qRT-PCR). Results: Low expression of CCAT1 was identified in normal gastric mucosa samples obtained from morbidly obese patients [mean Relative Quantity (RQ) = 1.95±0.4]. AGS human gastric carcinoma cell line showed an elevated level of CCAT1 expression (RQ=8.02). Expression levels of CCAT1 were approximately 10.8 fold higher in GC samples than in samples taken from the negative control group (RQ=21.1±5 vs. RQ=1.95±0.4, respectively, p<0.001). Interestingly, CCAT1 expression was significantly overexpressed in adjacent normal tissues when compared to the negative control group (RQ = 15.25±2 vs. RQ=1.95±0.4, respectively, p<0.001). Tissues obtained from recurrent GC cases showed the highest expression levels (RQ = 88.8±31; p<0.001). Expression levels increased with tumor stage (T4- 36.4±15, T3- 16.1±6, T2- 4.7±1), however this did not reach statistical significance (p=0.2). There was no difference in CCAT1 expression between intestinal and diffuse type GC (RQ=22.4±7 vs. 22.4±16, respectively, p=0.9). Within the normal gastric tissue samples, no significant difference in CCAT1 expression was observed in helicobacter pylori negative and positive patients (RQ= 2.4±0.9 vs. 0.93±0.2, respectively, p=0.13). Conclusion: CCAT1 is up-regulated in gastric cancer, and may serve as a potential bio-marker for early detection and surveillance.en_US
dc.identifier.doi10.7150/jca.10568en_US
dc.identifier.issn1837-9664
dc.identifier.urihttp://hdl.handle.net/11693/23392
dc.language.isoEnglishen_US
dc.publisherIvyspring International Publisheren_US
dc.relation.isversionofhttp://dx.doi.org/10.7150/jca.10568en_US
dc.source.titleJournal of Canceren_US
dc.subjectBiomarkeren_US
dc.subjectCCAT1en_US
dc.subjectGastrectomyen_US
dc.subjectGastric canceren_US
dc.subjectLong non-coding RNAen_US
dc.subjectColon cancer associated transcript 1en_US
dc.subjectLong untranslated RNAen_US
dc.subjectUnclassified drugen_US
dc.subjectAdulten_US
dc.subjectAgeden_US
dc.subjectArticleen_US
dc.subjectCancer stagingen_US
dc.subjectCancer surgeryen_US
dc.subjectCarcinoma cell lineen_US
dc.subjectClinical articleen_US
dc.subjectControlled studyen_US
dc.subjectFemaleen_US
dc.subjectGene expressionen_US
dc.subjectGene overexpressionen_US
dc.subjectHelicobacter pylorien_US
dc.subjectHumanen_US
dc.subjectHuman tissueen_US
dc.subjectLaparoscopic surgeryen_US
dc.subjectMaleen_US
dc.subjectMiddle ageden_US
dc.subjectMorbid obesityen_US
dc.subjectQuantitative analysisen_US
dc.subjectReal time polymerase chain reactionen_US
dc.subjectRNA extractionen_US
dc.subjectSleeve gastrectomyen_US
dc.subjectStomach adenocarcinomaen_US
dc.subjectStomach carcinomaen_US
dc.subjectStomach mucosaen_US
dc.subjectVery elderlyen_US
dc.titleColon cancer associated transcript-1 (CCAT1) expression in adenocarcinoma of the stomachen_US
dc.typeArticleen_US

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