High-throughput, high-resolution interferometric light microscopy of biological nanoparticles
buir.contributor.author | Özbay, Ekmel | |
buir.contributor.author | Ünlü, M. Selim | |
buir.contributor.orcid | Özbay, Ekmel|0000-0003-2953-1828 | |
dc.citation.epage | 2013 | en_US |
dc.citation.issueNumber | 22 | en_US |
dc.citation.spage | 2002 | en_US |
dc.citation.volumeNumber | 14 | en_US |
dc.contributor.author | Yurdakul, C. | |
dc.contributor.author | Avcı, O. | |
dc.contributor.author | Matlock, A. | |
dc.contributor.author | Devaux, A. J. | |
dc.contributor.author | Quintero, M. V. | |
dc.contributor.author | Özbay, Ekmel | |
dc.contributor.author | Davey, R. A. | |
dc.contributor.author | Connor, J. H. | |
dc.contributor.author | Karl, W. C. | |
dc.contributor.author | Tian, L. | |
dc.contributor.author | Ünlü, M. Selim | |
dc.date.accessioned | 2021-02-12T11:56:32Z | |
dc.date.available | 2021-02-12T11:56:32Z | |
dc.date.issued | 2020-01 | |
dc.department | Department of Electrical and Electronics Engineering | en_US |
dc.department | Department of Physics | en_US |
dc.department | Institute of Materials Science and Nanotechnology (UNAM) | en_US |
dc.department | Nanotechnology Research Center (NANOTAM) | en_US |
dc.description.abstract | Label-free, visible light microscopy is an indispensable tool for studying biological nanoparticles (BNPs). However, conventional imaging techniques have two major challenges: (i) weak contrast due to low-refractive-index difference with the surrounding medium and exceptionally small size and (ii) limited spatial resolution. Advances in interferometric microscopy have overcome the weak contrast limitation and enabled direct detection of BNPs, yet lateral resolution remains as a challenge in studying BNP morphology. Here, we introduce a wide-field interferometric microscopy technique augmented by computational imaging to demonstrate a 2-fold lateral resolution improvement over a large field-of-view (>100 × 100 μm2 ), enabling simultaneous imaging of more than 104 BNPs at a resolution of ∼150 nm without any labels or sample preparation. We present a rigorous vectorial-optics-based forward model establishing the relationship between the intensity images captured under partially coherent asymmetric illumination and the complex permittivity distribution of nanoparticles. We demonstrate high-throughput morphological visualization of a diverse population of Ebola virus-like particles and a structurally distinct Ebola vaccine candidate. Our approach offers a low-cost and robust label-free imaging platform for high-throughput and high-resolution characterization of a broad size range of BNPs. | en_US |
dc.description.provenance | Submitted by Evrim Ergin (eergin@bilkent.edu.tr) on 2021-02-12T11:56:32Z No. of bitstreams: 1 High-throughput_high-resolution_interferometric_light_microscopy_of_biological_nanoparticles.pdf: 5342371 bytes, checksum: 139fffe1f1605f3d9ce9d09e0b4eb8bd (MD5) | en |
dc.description.provenance | Made available in DSpace on 2021-02-12T11:56:32Z (GMT). No. of bitstreams: 1 High-throughput_high-resolution_interferometric_light_microscopy_of_biological_nanoparticles.pdf: 5342371 bytes, checksum: 139fffe1f1605f3d9ce9d09e0b4eb8bd (MD5) Previous issue date: 2020-01 | en |
dc.identifier.doi | 10.1021/acsnano.9b08512 | en_US |
dc.identifier.issn | 1936-0851 | |
dc.identifier.uri | http://hdl.handle.net/11693/55118 | |
dc.language.iso | English | en_US |
dc.publisher | American Chemical Society | en_US |
dc.relation.isversionof | https://dx.doi.org/10.1021/acsnano.9b08512 | en_US |
dc.source.title | ACS Nano | en_US |
dc.subject | Interference microscopy | en_US |
dc.subject | Nanoparticle detection | en_US |
dc.subject | Biosensing | en_US |
dc.subject | Computational imaging | en_US |
dc.subject | Label-free | en_US |
dc.subject | Fourier optics | en_US |
dc.title | High-throughput, high-resolution interferometric light microscopy of biological nanoparticles | en_US |
dc.type | Article | en_US |
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