Highly sensitive determination of 2, 4, 6-trinitrotoluene and related byproducts using a diol functionalized column for high performance liquid chromatography

buir.contributor.authorGüler, Mustafa O.
dc.citation.epage8en_US
dc.citation.issueNumber6en_US
dc.citation.spage1en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorGumuscu, B.en_US
dc.contributor.authorErdogan, Z.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, T.en_US
dc.date.accessioned2016-02-08T10:53:05Z
dc.date.available2016-02-08T10:53:05Z
dc.date.issued2014en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractIn this work, a new detection method for complete separation of 2,4,6-trinitrotoluene (TNT); 2,4-dinitrotoluene (2,4-DNT); 2,6-dinitrotoluene (2,6-DNT); 2-aminodinitrotoluene (2-ADNT) and 4-aminodinitrotoluene (4-ADNT) molecules in high-performance liquid-chromatography (HPLC) with UV sensor has been developed using diol column. This approach improves on cost, time, and sensitivity over the existing methods, providing a simple and effective alternative. Total analysis time was less than 13 minutes including column re-equilibration between runs, in which water and acetonitrile were used as gradient elution solvents. Under optimized conditions, the minimum resolution between 2,4-DNT and 2,6-DNT peaks was 2.06. The recovery rates for spiked environmental samples were between 95-98%. The detection limits for diol column ranged from 0.78 to 1.17 μg/L for TNT and its byproducts. While the solvent consumption was 26.4 mL/min for two-phase EPA and 30 mL/min for EPA 8330 methods, it was only 8.8 mL/min for diol column. The resolution was improved up to 49% respect to two-phase EPA and EPA 8330 methods. When compared to C-18 and phenyl-3 columns, solvent usage was reduced up to 64% using diol column and resolution was enhanced approximately two-fold. The sensitivity of diol column was afforded by the hydroxyl groups on polyol layer, joining the formation of charge-transfer complexes with nitroaromatic compounds according to acceptor-donor interactions. Having compliance with current requirements, the proposed method demonstrates sensitive and robust separation. © 2014 Gumuscu et al.en_US
dc.description.provenanceMade available in DSpace on 2016-02-08T10:53:05Z (GMT). No. of bitstreams: 1 bilkent-research-paper.pdf: 70227 bytes, checksum: 26e812c6f5156f83f0e77b261a471b5a (MD5) Previous issue date: 2014en
dc.identifier.doi10.1371/journal.pone.0099230en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11693/25969
dc.language.isoEnglishen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttps://doi.org/10.1371/journal.pone.0099230en_US
dc.source.titlePLOS ONEen_US
dc.subject2 aminodinitrotolueneen_US
dc.subject2,4 dinitrotolueneen_US
dc.subject2,6 dinitrotolueneen_US
dc.subject4 aminodinitrotolueneen_US
dc.subjectAcetonitrileen_US
dc.subjectAromatic nitro compounden_US
dc.subjectHydroxyl groupen_US
dc.subjectTrinitrotolueneen_US
dc.subjectUnclassified drugen_US
dc.subjectWateren_US
dc.subjectChemical analysisen_US
dc.subjectControlled studyen_US
dc.subjectEnvironmental monitoringen_US
dc.subjectHigh performance liquid chromatographyen_US
dc.subjectLimit of detectionen_US
dc.subjectMolecular interactionen_US
dc.subjectProcess optimizationen_US
dc.subjectSensitivity analysisen_US
dc.subjectUltraviolet radiationen_US
dc.titleHighly sensitive determination of 2, 4, 6-trinitrotoluene and related byproducts using a diol functionalized column for high performance liquid chromatographyen_US
dc.typeArticleen_US

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