An optofluidic point-of-care device for quantitative investigation of erythrocyte aggregation during coagulation

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buir.contributor.authorIşıksaçan, Ziya
buir.contributor.authorHastar, Nurcan
buir.contributor.authorElbüken, Çağlar
dc.citation.epage30en_US
dc.citation.spage24en_US
dc.citation.volumeNumber281en_US
dc.contributor.authorIşıksaçan, Ziyaen_US
dc.contributor.authorHastar, Nurcanen_US
dc.contributor.authorErel, Ö.en_US
dc.contributor.authorElbüken, Çağlaren_US
dc.date.accessioned2019-02-21T16:02:00Z
dc.date.available2019-02-21T16:02:00Z
dc.date.issued2018en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractCoagulation, the process leading to clot formation with the interplay of blood constituents, is a self-regulating mechanism, requiring attentive and periodic monitoring for numerous clinical cases. Erythrocyte aggregation (EA) is a characteristic behaviour of erythrocytes forming reversible clumps especially in vitro at low shear rates. The effect of EA during coagulation is overlooked in whole blood (WB) clotting assays, and the relationship between the two mechanisms is not well understood. We present an optofluidic point-of-care device enabling quantitative investigation of EA from 50 μl WB during the coagulation process. Not only did we explain the coagulation mechanism considering EA, but we also demonstrated coagulation time measurement from optical EA analysis. The device consists of a disposable cartridge and a handheld analyzer containing a pinch valve for fluid motion and optics for transmitted light measurement. Following the sample introduction and cessation of the valve operation, the optical signal is the lowest due to shear-induced cell disaggregation. Then, the signal increases due to EA until reaching a peak, indicating blood clotting. The working principle was proven through clinical tests for prothrombin time measurement. In addition to revealing the relation between coagulation and aggregation, this device is promising for rapid WB coagulation time measurement.
dc.description.sponsorshipThe authors thank Hazal Isiksacan, Abtin Saateh, Alper Demir, and Pinar Beyazkilic for their help in figures, fluorescence microscopy, clinical tests, and manuscript proofreading, respectively. Ziya Isiksacan is supported by ASELSAN Graduate Scholarship for Turkish Academicians . The authors acknowledge support from The Scientific and Technologic Research Council of Turkey (TUBITAK project no. 213S127).
dc.embargo.release2020-10-01en_US
dc.identifier.doi10.1016/j.sna.2018.08.007
dc.identifier.issn0924-4247
dc.identifier.urihttp://hdl.handle.net/11693/49949
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.relation.isversionofhttps://doi.org/10.1016/j.sna.2018.08.007
dc.relation.projectASELSAN - 213S127
dc.source.titleSensors and Actuators, A: Physicalen_US
dc.subjectCoagulationen_US
dc.subjectErythrocyte aggregationen_US
dc.subjectFibrinogenen_US
dc.subjectLab-on-a-chipen_US
dc.subjectOptofluidicen_US
dc.titleAn optofluidic point-of-care device for quantitative investigation of erythrocyte aggregation during coagulationen_US
dc.typeArticleen_US

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