Genetic circuits to detect nanomaterial triggered toxicity through engineered heat shock response mechanism

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buir.contributor.authorSaltepe, Behide
buir.contributor.authorBozkurt, Eray Ulaş
buir.contributor.authorHacıosmanoğlu, Nedim
buir.contributor.authorŞeker, Urartu Özgür Şafak
dc.citation.epage2417en_US
dc.citation.issueNumber10en_US
dc.citation.spage2404en_US
dc.citation.volumeNumber8en_US
dc.contributor.authorSaltepe, Behideen_US
dc.contributor.authorBozkurt, Eray Ulaşen_US
dc.contributor.authorHacıosmanoğlu, Nedimen_US
dc.contributor.authorŞeker, Urartu Özgür Şafaken_US
dc.date.accessioned2020-02-12T11:20:27Z
dc.date.available2020-02-12T11:20:27Z
dc.date.issued2019
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.description.abstractBiocompatibility assessment of nanomaterials has been of great interest due to their potential toxicity. However, conventional biocompatibility tests fall short of providing a fast toxicity report. We developed a whole cell based biosensor to track biocompatibility of nanomaterials with the aim of providing fast feedback to engineer them with lower toxicity levels. We engineered promoters of four heat shock response (HSR) proteins utilizing synthetic biology approaches. As an initial design, a reporter coding gene was cloned downstream of the selected promoter regions. Initial results indicated that native heat shock protein (HSP) promoter regions were not very promising to generate signals with low background signals. Introducing riboregulators to native promoters eliminated unwanted background signals almost entirely. Yet, this approach also led to a decrease in expected sensor signal upon stress treatment. Thus, a repression based genetic circuit, inspired by the HSR mechanism of Mycobacterium tuberculosis, was constructed. These genetic circuits could report the toxicity of quantum dot nanoparticles in 1 h. Our designed nanoparticle toxicity sensors can provide quick reports, which can lower the demand for additional experiments with more complex organisms.en_US
dc.identifier.doi10.1021/acssynbio.9b00291en_US
dc.identifier.issn2161-5063
dc.identifier.urihttp://hdl.handle.net/11693/53305
dc.language.isoEnglishen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttps://dx.doi.org/10.1021/acssynbio.9b00291en_US
dc.source.titleACS Synthetic Biologyen_US
dc.subjectNanotoxicityen_US
dc.subjectNanomaterial triggered toxicityen_US
dc.subjectHeat shock responseen_US
dc.subjectSynthetic biologyen_US
dc.subjectWhole-cell biosensorsen_US
dc.titleGenetic circuits to detect nanomaterial triggered toxicity through engineered heat shock response mechanismen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)
Nanotechnology Research Center (NANOTAM)