Screening and selection of novel animal probiotics isolated from bovine chyme

Date
2013
Authors
Ozkan, A.D.
Han, D.
Umu O.C.O.
Angun P.
Senturk, B.
Yasa O.
Tekinay, T.
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Annals of Microbiology
Print ISSN
15904261
Electronic ISSN
Publisher
Volume
63
Issue
4
Pages
1291 - 1300
Language
English
Type
Article
Journal Title
Journal ISSN
Volume Title
Series
Abstract

Probiotics, gut-colonizing microorganisms capable of conferring a number of health benefits to their hosts, are highly desirable as animal feed supplements. Members of the Gram-positive genus Bacillus are often utilized as probiotics, since endospores formed by those bacteria render them highly resistant to environmental extremes and therefore capable of surviving gastrointestinal tract conditions. In this study, 84 distinct bacterial colonies were obtained from bovine chyme and 29 isolates were determined as Bacillus species. These isolates were principally screened for their antimicrobial activity against a group of two Gram-positive and fourGram-negative bacteria, including known human and animal pathogens such as Salmonella enterica, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Seven strains displaying strong antimicrobial activity against the test cohort were further evaluated for other properties desirable from animal probiotics, including high spore-forming capacity and adhesiveness, resistance to pH extremes and ability to form biofilms. The isolates were found to resist simulated gastrointestinal conditions and most of the antibiotics tested. In addition, plasmid presence was checked and cytotoxicity tests were performed to evaluate the potential risks of antibiotic resistance transfer and unintended pathogenic effects on host, respectively. We propose that the bacterial isolates are suitable for use as animal probiotics. © Springer-Verlag Berlin Heidelberg and the University of Milan 2012.

Course
Other identifiers
Book Title
Keywords
Antimicrobial, Bacillus, Feed supplement, Isolation, Probiotics, Animalia, Bacillus (bacterium), Bacteria (microorganisms), Bovinae, Klebsiella pneumoniae, Posibacteria, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus
Citation
Published Version (Please cite this version)