Protective therapeutic effects of peptide nanofiber and hyaluronic acid hybrid membrane in in vivo osteoarthritis model

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buir.contributor.authorArslan, Elif
buir.contributor.authorEkiz, Melis Sardan
buir.contributor.authorCimenci, Cagla Eren
buir.contributor.authorGüler, Mustafa O.
buir.contributor.authorTekinay, Ayse B.
dc.citation.epage274en_US
dc.citation.spage263en_US
dc.citation.volumeNumber73en_US
dc.contributor.authorArslan, Elifen_US
dc.contributor.authorEkiz, Melis Sardanen_US
dc.contributor.authorCimenci, Cagla Erenen_US
dc.contributor.authorCan, N.en_US
dc.contributor.authorGemci, M. H.en_US
dc.contributor.authorOzkan, H.en_US
dc.contributor.authorGüler, Mustafa O.en_US
dc.contributor.authorTekinay, Ayse B.en_US
dc.date.accessioned2019-02-21T16:01:22Z
dc.date.available2019-02-21T16:01:22Z
dc.date.issued2018en_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentInterdisciplinary Program in Neuroscience (NEUROSCIENCE)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.description.abstractOsteoarthritis (OA) is a condition where tissue function is lost through a combination of secondary inflammation and deterioration in articular cartilage. One of the most common causes of OA is age-related tissue impairment because of wear and tear due to mechanical erosion. Hyaluronic acid-based viscoelastic supplements have been widely used for the treatment of knee injuries. However, the current formulations of hyaluronic acid are unable to provide efficient healing and recovery. Here, a nanofiber-hyaluronic acid membrane system that was prepared by using a quarter of the concentration of commercially available hyaluronic acid supplement, Hyalgan® was used for the treatment of an osteoarthritis model, and Synvisc® which is another commercially available hyaluronic acid containing viscoelastic supplement, was used as a control. The results show that this system provides efficient protection of arthritic cartilage tissue through the preservation of cartilage morphology with reduced osteophyte formation, protection of the subchondral region from deterioration, and maintenance of cartilage specific matrix proteins in vivo. In addition, the hybrid nanofiber membrane enabled chondrocyte encapsulation and provided a suitable culturing environment for stem cell growth in vitro. Overall, our results suggest that this hybrid nanofibrous scaffold provides a potential platform the treatment of OA. Statement of Significance: Osteoarthritis is a debilitating joint disease affecting millions of people worldwide. It occurs especially in knees due to aging, sport injuries or obesity. Although hyaluronic acid-based viscoelastic supplements are widely used, there is still no effective treatment method for osteoarthritis, which necessitates surgical operation as an only choice for severe cases. Therefore, there is an urgent need for efficient therapeutics. In this study, a nanofiber-HA membrane system was developed for the efficient protection of arthritic cartilage tissue from degeneration. This hybrid nanofiber system provided superior therapeutic activity at a relatively lower concentration of hyaluronic acid than Hyalgan® and Synvisc® gels, which are currently used in clinics. This work demonstrates for the first time that this hybrid nanofiber membrane scaffold can be utilized as a potential candidate for osteoarthritis treatment.
dc.description.sponsorshipE. A. acknowledges support from TUBITAK-BIDEB fellowship. We would like to express our gratitude to Mr. M. Guler for assistance in TEM, to veterinarian Mr. M. Ak for assistance in in vivo experiments, and to Dr. A. D. Ozkan and Dr. B. Senturk for valuable comments. A.B.T. acknowledges support from the Science Academy Outstanding Young Scientist Award (BAGEP).
dc.identifier.doi10.1016/j.actbio.2018.04.015
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/11693/49830
dc.language.isoEnglish
dc.publisherActa Materialia Inc
dc.relation.isversionofhttps://doi.org/10.1016/j.actbio.2018.04.015
dc.relation.projectTyö- ja Elinkeinoministeriö, TEM
dc.source.titleActa Biomaterialiaen_US
dc.subjectHyaluronic aciden_US
dc.subjectHybrid membraneen_US
dc.subjectOsteoarthritis modelen_US
dc.subjectPeptide gelen_US
dc.subjectProtectionen_US
dc.titleProtective therapeutic effects of peptide nanofiber and hyaluronic acid hybrid membrane in in vivo osteoarthritis modelen_US
dc.typeArticleen_US

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Institute of Materials Science and Nanotechnology (UNAM)
Aysel Sabuncu Brain Research Center (BAM)
Nanotechnology Research Center (NANOTAM)