Label-free identification of exosomes using raman spectroscopy and machine learning

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buir.contributor.authorInci, Fatih
buir.contributor.orcidInci, Fatih|0000-0002-9918-5038
dc.citation.epage2205519-12en_US
dc.citation.issueNumber9
dc.citation.spage2205519-1
dc.citation.volumeNumber19
dc.contributor.authorParlatan, U.
dc.contributor.authorOzen, M.O.
dc.contributor.authorKecoglu, I.
dc.contributor.authorKoyuncu, B.
dc.contributor.authorTorun, H.
dc.contributor.authorKhalafkhany, D.
dc.contributor.authorLoc, I.
dc.contributor.authorOgut, M.G.
dc.contributor.authorInci, Fatih
dc.contributor.authorAkin, D.
dc.contributor.authorSolaroglu, I.
dc.contributor.authorOzoren, N.
dc.contributor.authorUnlu, M. B.
dc.contributor.authorDemirci, U.
dc.date.accessioned2024-03-09T07:31:18Z
dc.date.available2024-03-09T07:31:18Z
dc.date.issued2023-01-15
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)
dc.description.abstractExosomes, nano-sized extracellular vesicles (EVs) secreted from cells, carry various cargo molecules reflecting their cells of origin. As EV content, structure, and size are highly heterogeneous, their classification via cargo molecules by determining their origin is challenging. Here, a method is presented combining surface-enhanced Raman spectroscopy (SERS) with machine learning algorithms to employ the classification of EVs derived from five different cell lines to reveal their cellular origins. Using an artificial neural network algorithm, it is shown that the label-free Raman spectroscopy method's prediction ratio correlates with the ratio of HT-1080 exosomes in the mixture. This machine learning-assisted SERS method enables a new direction through label-free investigation of EV preparations by differentiating cancer cell-derived exosomes from those of healthy. This approach will potentially open up new avenues of research for early detection and monitoring of various diseases, including cancer.
dc.identifier.doi10.1002/smll.202205519
dc.identifier.eissn1613-6829
dc.identifier.issn1613-6810
dc.identifier.urihttps://hdl.handle.net/11693/114436
dc.language.isoen
dc.publisherWiley-VCH Verlag GmbH & Co. KGaA
dc.relation.isversionofhttps://doi.org/10.1002/smll.202205519
dc.rights.licenseCC BY
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.source.titleSmall
dc.subjectExosome
dc.subjectExtracellular vesicles
dc.subjectNeural networks
dc.subjectRaman spectroscopy
dc.titleLabel-free identification of exosomes using raman spectroscopy and machine learning
dc.typeArticle

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Institute of Materials Science and Nanotechnology (UNAM)