Compartmentalizing and sculpting nanovesicles by phase-separated aqueous nanodroplets

Simple item page
buir.contributor.authorBahrami, Amir Houshang
buir.contributor.orcidBahrami, Amir Houshang|0000-0001-5841-2516
dc.citation.epage32045en_US
dc.citation.issueNumber49en_US
dc.citation.spage32035en_US
dc.citation.volumeNumber12en_US
dc.contributor.authorSabet, Fatemeh Kazemi
dc.contributor.authorBahrami, Arash
dc.contributor.authorBahrami, Amir Houshang
dc.date.accessioned2023-03-01T06:16:43Z
dc.date.available2023-03-01T06:16:43Z
dc.date.issued2022-11-08
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.description.abstractPhase-separated liquid droplets inside giant vesicles have been intensely studied as biomimetic model systems to understand cellular microcompartmentation and molecular crowding and sorting. On the nanoscale, however, how aqueous nanodroplets interact with and shape nanovesicles is poorly understood. We perform coarse-grained molecular simulations to explore the architecture of compartmentalized nanovesicles by phase-separated aqueous nanodroplets, and their morphological evolution under osmotic deflation. We show that phase separation of a biphasic liquid mixture can form both stable two-compartment and meta-stable multi-compartment nanovesicles. We identify morphological transitions of stable two-compartment nanovesicles between tube, sheet and cup morphologies, characterized by membrane asymmetry and phase-separation propensity between the aqueous phases. We demonstrate that the formation of local sheets and in turn cup-shaped nanovesicles is promoted by negative line tensions resulting from large separation propensities, an exclusive nanoscale phenomenon which is not expected for larger vesicles where energetic contributions of the line tensions are dominated by those of the membrane tensions. Despite their instability, we observe long-lived multi-compartment nanovesicles, such as nanotubules and branched tubules, whose prolonged lifetime is attributed to interfacial tensions and membrane asymmetry. Aqueous nanodroplets can thus form novel membrane nanostructures, crucial for cellular processes and forming cellular organelles on the nanoscale. © 2022 The Royal Society of Chemistry.en_US
dc.description.provenanceSubmitted by Cem Çağatay Akgün (cem.akgun@bilkent.edu.tr) on 2023-03-01T06:16:43Z No. of bitstreams: 1 Hand_explorations_are_determined_by_the_characteristics_of_the_perceptual_space_of_real-world_materials_from_silk_to_sand.pdf: 1709024 bytes, checksum: 4b81aebd98a3d9225c8349f002d7e71a (MD5)en
dc.description.provenanceMade available in DSpace on 2023-03-01T06:16:43Z (GMT). No. of bitstreams: 1 Hand_explorations_are_determined_by_the_characteristics_of_the_perceptual_space_of_real-world_materials_from_silk_to_sand.pdf: 1709024 bytes, checksum: 4b81aebd98a3d9225c8349f002d7e71a (MD5) Previous issue date: 2022-11-08en
dc.identifier.doi10.1039/d2ra05855cen_US
dc.identifier.issn20462069
dc.identifier.urihttp://hdl.handle.net/11693/111979
dc.language.isoEnglishen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionofhttps://dx.doi.org/10.1039/d2ra05855cen_US
dc.source.titleRSC Advancesen_US
dc.subjectBiomimeticsen_US
dc.subjectCoarse-grained modelingen_US
dc.subjectMembranesen_US
dc.subjectNanotechnologyen_US
dc.titleCompartmentalizing and sculpting nanovesicles by phase-separated aqueous nanodropletsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Compartmentalizing_and_sculpting_nanovesicles_by_phase-separated_aqueous_nanodroplets.pdf
Size:
11.34 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.69 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Scholarly Publications - UNAM