CpG loaded flourescent polymeric nanoparticles: a theranostic drug delivery system suitable for TLR based therapies

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dc.contributor.authorKahraman, Tameren_US
dc.contributor.authorBayyurt, Banuen_US
dc.contributor.authorİbrahimova, Vuselaen_US
dc.contributor.authorTuncel, Dönüşen_US
dc.contributor.authorGürsel, İhsanen_US
dc.coverage.spatialAntalya, Turkeyen_US
dc.date.accessioned2019-07-05T11:23:37Z
dc.date.available2019-07-05T11:23:37Z
dc.date.issued2012-04en_US
dc.departmentDepartment of Chemistryen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.descriptionConference Name: Molecular Immunology & Immunogenetics Congress, MIMIC 2012en_US
dc.descriptionDate of Conference: 27-29 April 2012en_US
dc.description.abstractDesigning nanoparticulate delivery systems suitable for simultaneous imaging have been attracting great interest. Several systems such as liposomes, micelles, dendrimers, nanospheres and nanocapsules are potential theranostic carriers in biomedical applications. Here, for the first time we describe a complexation strategy allowing us to deliver a TLR ligand along with simultaneous imaging of the tissues with fluorescent polymeric nanoparticles (NPs). METHOD: Four different NPs (P1, P2, P3 and P4) were used for biocompatibility and drug delivery experiments. RAW264.7 cells were incubated with NPs for indicated time periods and analyzed by FACS and confocal microscopy. Cytotoxicity experiments were performed with Dojindo reagent on RAW264.7 cells. In addition, cellular uptake of NPs in the presence of several scavenger receptor ligands was also investigated. Next, NPs were incubated with CpG ODN to yield nanocomplexes. In vitro and in vivo immunostimulatory effect of nanocomplexes was analyzed both on PBMCs or splenocytes. Pro-inflammatory cytokine production was assessed either by ELISA or in some cases by PCR.en_US
dc.description.provenanceSubmitted by Zeynep Aykut (zeynepay@bilkent.edu.tr) on 2019-07-05T11:23:37Z No. of bitstreams: 1 W-Progress.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5)en
dc.description.provenanceMade available in DSpace on 2019-07-05T11:23:37Z (GMT). No. of bitstreams: 1 W-Progress.pdf: 179475 bytes, checksum: ea0bedeb05ac9ccfb983c327e155f0c2 (MD5) Previous issue date: 2012-04en
dc.identifier.urihttp://hdl.handle.net/11693/52128
dc.language.isoEnglishen_US
dc.publisherTurkish Society of Immunologyen_US
dc.source.titleMolecular Immunology & Immunogenetics Congress, MIMIC 2012en_US
dc.titleCpG loaded flourescent polymeric nanoparticles: a theranostic drug delivery system suitable for TLR based therapiesen_US
dc.typeConference Paperen_US

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